Previous Article | Next Article 
Journal of Virology, October 2001, p. 9121-9128, Vol. 75, No. 19
Department of Veterinary Public Health,
Faculty of Agriculture, Gifu University, Gifu 501-1193, Japan
Received 2 March 2001/Accepted 28 June 2001
In order to identify the viral gene related to the pathogenicity of
rabies virus, we tried to establish a reverse genetics system of the
attenuated RC-HL strain, which causes nonlethal infection in adult mice
after intracerebral inoculation. A full-length genome plasmid encoding
the complete antigenomic cDNA of the RC-HL strain and helper plasmids
containing cDNAs of the complete open reading frame of the N, P, and L
genes, respectively, were constructed. After transfection of these
plasmids into BHK-21 cells infected with the T7 RNA
polymerase-expressing vaccinia virus, infectious rabies virus with
almost the same biological properties as those of the wild-type RC-HL
strain was rescued. Using this reverse genetics system of the RC-HL
strain, we generated a chimeric virus with the open reading frame of
the glycoprotein gene from the parent Nishigahara strain,
which kills adult mice after intracerebral inoculation, in the
background of the RC-HL genome. Since the chimeric virus killed adult
mice following intracerebral inoculation, it became evident that the
open reading frame of the glycoprotein gene is related to
the pathogenicity of the Nishigahara strain for adult mice.
0022-538X/01/$04.00+0 DOI: 10.1128/JVI.75.19.9121-9128.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
Rescue of Rabies Virus from Cloned cDNA and Identification of the
Pathogenicity-Related Gene: Glycoprotein Gene Is Associated with
Virulence for Adult Mice
*
Corresponding author. Mailing address: Department of
Veterinary Public Health, Faculty of Agriculture, Gifu University,
Yanagido 1-1, Gifu 501-1193, Japan. Phone and fax: 81-58-293-2948. E-mail: minamoto{at}cc.gifu-u.ac.jp.
Journal of Virology, October 2001, p. 9121-9128, Vol. 75, No. 19
0022-538X/01/$04.00+0 DOI: 10.1128/JVI.75.19.9121-9128.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
This article has been cited by other articles:
-
Pulmanausahakul, R., Li, J., Schnell, M. J., Dietzschold, B.
(2008). The Glycoprotein and the Matrix Protein of Rabies Virus Affect Pathogenicity by Regulating Viral Replication and Facilitating Cell-to-Cell Spread. J. Virol.
82: 2330-2338
[Abstract] [Full Text] -
Faber, M., Faber, M.-L., Papaneri, A., Bette, M., Weihe, E., Dietzschold, B., Schnell, M. J.
(2005). A Single Amino Acid Change in Rabies Virus Glycoprotein Increases Virus Spread and Enhances Virus Pathogenicity. J. Virol.
79: 14141-14148
[Abstract] [Full Text] -
Kassis, R., Larrous, F., Estaquier, J., Bourhy, H.
(2004). Lyssavirus Matrix Protein Induces Apoptosis by a TRAIL-Dependent Mechanism Involving Caspase-8 Activation. J. Virol.
78: 6543-6555
[Abstract] [Full Text] -
Martinez, I., Rodriguez, L. L., Jimenez, C., Pauszek, S. J., Wertz, G. W.
(2003). Vesicular Stomatitis Virus Glycoprotein Is a Determinant of Pathogenesis in Swine, a Natural Host. J. Virol.
77: 8039-8047
[Abstract] [Full Text] -
Guyatt, K. J., Twin, J., Davis, P., Holmes, E. C., Smith, G. A., Smith, I. L., Mackenzie, J. S., Young, P. L.
(2003). A molecular epidemiological study of Australian bat lyssavirus. J. Gen. Virol.
84: 485-496
[Abstract] [Full Text] -
Neumann, G., Whitt, M. A., Kawaoka, Y.
(2002). A decade after the generation of a negative-sense RNA virus from cloned cDNA - what have we learned?. J. Gen. Virol.
83: 2635-2662
[Abstract] [Full Text]
Copyright © 2009 by the American Society for Microbiology. For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»