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Journal of Virology, September 2001, p. 8854-8858, Vol. 75, No. 18
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.18.8854-8858.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Varicella-Zoster Virus ORF47 Protein Serine Kinase: Characterization of a Cloned, Biologically Active Phosphotransferase and Two Viral Substrates, ORF62 and ORF63

T. K. Kenyon,1 J. Lynch,2 J. Hay,2 W. Ruyechan,2 and C. Grose1,*

Departments of Microbiology and Pediatrics, University of Iowa, Iowa City, Iowa,1 and Department of Microbiology, State University of New York, Buffalo, New York2

Received 24 April 2001/Accepted 11 June 2001

Varicella-zoster virus (VZV) codes for a protein serine kinase called ORF47; the herpes simplex virus (HSV) homolog is UL13. No recombinant alphaherpesvirus serine kinase has been biologically active in vitro. We discovered that preservation of the intrinsic kinase activity of recombinant VZV ORF47 required unusually stringent in vitro conditions, including physiological concentrations of polyamines. In this assay, ORF47 phosphorylated two VZV regulatory proteins: the ORF62 protein (homolog of HSV ICP4) and the ORF63 protein (homolog of HSV ICP22). Of interest, ORF47 kinase also coprecipitated ORF63 protein from the kinase assay supernatant.

* Corresponding author. Mailing address: University Hospital, 2501 JCP, 200 Hawkins Dr., Iowa City, IA 52242. Phone: (319) 356-2270. Fax: (319) 356-4855. E-mail: charles-grose{at}uiowa.edu.

Journal of Virology, September 2001, p. 8854-8858, Vol. 75, No. 18
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.18.8854-8858.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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