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Journal of Virology, September 2001, p. 8283-8288, Vol. 75, No. 17
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.17.8283-8288.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Latent Antigen Vaccination in a Model Gammaherpesvirus Infection

Edward J. Usherwood,1,dagger Kimberley A. Ward,1,Dagger Marcia A. Blackman,1 James P. Stewart,2 and David L. Woodland1,*

The Trudeau Institute, Saranac Lake, New York 12983,1 and The Laboratory for Clinical and Molecular Virology, Department of Veterinary Pathology, The University of Edinburgh, Edinburgh EH9 1QH, United Kingdom2

Received 3 April 2001/Accepted 4 June 2001

Vaccines that can reduce the load of latent gammaherpesvirus infections are eagerly sought. One attractive strategy is vaccination against latency-associated proteins, which may increase the efficiency with which T cells recognize and eliminate latently infected cells. However, due to the lack of tractable animal model systems, the effect of latent-antigen vaccination on gammaherpesvirus latency is not known. Here we use the murine gammaherpesvirus model to investigate the impact of vaccination with the latency-associated M2 antigen. As expected, vaccination had no effect on the acute lung infection. However, there was a significant reduction in the load of latently infected cells in the initial stages of the latent infection, when M2 is expressed. These data show for the first time that latent-antigen vaccination can reduce the level of latency in vivo and suggest that vaccination strategies involving other latent antigens may ultimately be successfully used to reduce the long-term latent infection.

* Corresponding author. Mailing address: The Trudeau Institute, 100 Algonquin Ave., Saranac Lake, NY 12983. Phone: (518) 891-3080, ext. 314. Fax: (518) 891-5126. E-mail: dwoodland{at}trudeauinstitute.org.

dagger Present address: Department of Microbiology and Immunology, Dartmouth Medical School, Lebanon, N.H.

Dagger Present address: University of Vermont, Department of Pathology, Burlington, VT 05405.

Journal of Virology, September 2001, p. 8283-8288, Vol. 75, No. 17
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.17.8283-8288.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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