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Journal of Virology, August 2001, p. 7763-7768, Vol. 75, No. 16
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.16.7763-7768.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Retroviral Splicing Suppressor Requires Three Nonconsensus Uridines in a 5' Splice Site-Like Sequence

Robert E. Paca, Catherine S. Hibbert,dagger Christopher T. O'Sullivan, and Karen L. Beemon*

Department of Biology, Johns Hopkins University, Baltimore, Maryland 21218

Received 6 March 2001/Accepted 21 May 2001

Rous sarcoma virus RNA contains a negative regulator of splicing (NRS) element that aids in maintenance of unspliced RNA. The NRS binds U1 snRNA at a sequence that deviates from the 5' splice site consensus by substitution of U's for A's at three positions: -2, +3, and +4. All three of these U's are important for NRS-mediated splicing suppression. Substitution of a single nonconsensus C or G at any of these sites diminished NRS activity, whereas substitution of a single A generated a preferred 5' splice site within the NRS.

* Corresponding author. Mailing address: Department of Biology, Johns Hopkins University, 3400 N. Charles St., Baltimore, MD 21218. Phone: (410) 516-7289. Fax: (410) 516-7292. E-mail: KLB{at}JHU.edu.

dagger Present address: NCI-Frederick Cancer Research and Development Center, Frederick, MD 21702.

Journal of Virology, August 2001, p. 7763-7768, Vol. 75, No. 16
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.16.7763-7768.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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