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Journal of Virology, August 2001, p. 7749-7755, Vol. 75, No. 16
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.16.7749-7755.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Physical and Functional Interactions between the Corepressor CtBP and the Epstein-Barr Virus Nuclear Antigen EBNA3C

Robert Touitou, Mark Hickabottom, Gillian Parker, Tim Crook, and Martin J. Allday*

Section of Virology and Cell Biology and Ludwig Institute for Cancer Research, Imperial College of Science, Technology and Medicine, St. Mary's Campus, London W2 1PG, United Kingdom

Received 30 January 2001/Accepted 22 May 2001

CtBP has been shown to be a highly conserved corepressor of transcription. E1A and all the various transcription factors to which CtBP binds contain a conserved PLDLS CtBP-interacting domain, and EBNA3C includes a PLDLS motif (amino acids [aa] 728 to 732). Here we show that EBNA3C binds to CtBP both in vitro and in vivo and that the interaction requires an intact PLDLS. The C terminus of EBNA3C (aa 580 to 992) has modest trans-repressor activity when it is fused to the DNA-binding domain of Gal4, and deletion or mutation of the PLDLS sequence ablates this and unmasks a transactivation function within the fragment. However, loss of the CtBP interaction motif had little effect on the ability of full-length EBNA3C to repress transcription. A striking correlation between CtBP binding and the capacity of EBNA3C to cooperate with (Ha-)Ras in the immortalization and transformation of primary rat embryo fibroblasts was also revealed.

* Corresponding author. Mailing address: Section of Virology and Cell Biology and Ludwig Institute for Cancer Research, Imperial College of Science, Technology and Medicine, St. Mary's Campus, Norfolk Place, London W2 1PG, United Kingdom. Phone: 44207 563 7724. Fax: 44207 724 8586. E-mail: m.allday{at}ic.ac.uk.

Journal of Virology, August 2001, p. 7749-7755, Vol. 75, No. 16
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.16.7749-7755.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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