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Journal of Virology, August 2001, p. 7739-7743, Vol. 75, No. 16
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.16.7739-7743.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Tetramerization Is a Conserved Feature of the Virion-Associated Protein in Plant Pararetroviruses

Livia Stavolone, Etienne Herzog, Denis Leclerc,dagger and Thomas Hohn*

Friedrich Miescher Institute, CH-4002 Basel, Switzerland

Received 22 March 2001/Accepted 7 May 2001

All plant pararetroviruses belong to the Caulimoviridae family. This family contains six genera of viruses with different biological, serological, and molecular characteristics. Although some important mechanisms of viral replication and host infection are understood, much remains to be discovered about the many functions of the viral proteins. The focus of this study, the virion-associated protein (VAP), is conserved among all members of the group and contains a coiled-coil structure that has been shown to assemble as a tetramer in the case of cauliflower mosaic virus. We have used the yeast two-hybrid system to characterize self-association of the VAPs of four distinct plant pararetroviruses, each belonging to a different genus of Caulimoviridae. Chemical cross-linking confirmed that VAPs assemble into tetramers. Tetramerization is thus a common property of these proteins in plant pararetroviruses. The possible implications of this conserved feature for VAP function are discussed.

* Corresponding author. Mailing address: Friedrich Miescher Institute, P.O. Box 2543, CH-4002 Basel, Switzerland. Phone: 41 61 6977266. Fax: 41 61 6973976. E-mail: hohn{at}fmi.ch.

dagger Present address: CHUQ, Centre de Recherche en Infectiologie, Quèbec, Quèbec G1V4G2, Canada.

Journal of Virology, August 2001, p. 7739-7743, Vol. 75, No. 16
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.16.7739-7743.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Stavolone, L., Villani, M. E., Leclerc, D., Hohn, T. (2005). A coiled-coil interaction mediates cauliflower mosaic virus cell-to-cell movement. Proc. Natl. Acad. Sci. USA 102: 6219-6224 [Abstract] [Full Text]  
  • Stavolone, L., Ragozzino, A., Hohn, T. (2003). Characterization of Cestrum yellow leaf curling virus: a new member of the family Caulimoviridae. J. Gen. Virol. 84: 3459-3464 [Abstract] [Full Text]  
  • Kobayashi, K., Tsuge, S., Stavolone, L., Hohn, T. (2002). The Cauliflower Mosaic Virus Virion-Associated Protein Is Dispensable for Viral Replication in Single Cells. J. Virol. 76: 9457-9464 [Abstract] [Full Text]  


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