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Journal of Virology, August 2001, p. 7175-7183, Vol. 75, No. 15
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.15.7175-7183.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Lipid Rafts and Pseudotyping

Winfried F. Pickl,dagger Felipe X. Pimentel-Muiños, and Brian Seed*

Department of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts 02114

Received 27 September 2000/Accepted 28 April 2001

Specific interactions between envelope and core proteins govern the membrane assembly of most enveloped viruses. Despite this, mixed infections lead to pseudotyping, the association of the viral cores of one virus with the envelopes of another. How does this occur? We show here that the detergent-insoluble lipid rafts of the plasma membrane function as a natural meeting point for the transmembrane and core components of a phylogenetically diverse collection of enveloped viruses. As a result, viral particles preferentially incorporate both the envelope components of other viruses as well as the extra- and intracellular constituents of host cell lipid rafts, including gangliosides, glycosyl phosphatidylinositol-anchored surface proteins, and intracellular signal transduction molecules. Pharmacological disruption of lipid rafts interferes with virus production.

* Corresponding author. Mailing address: Department of Molecular Biology, Massachusetts General Hospital, Boston, MA 02114. Phone: (617) 726-5975. Fax: (617) 726-6893. E-mail: seed{at}molbio.mgh.harvard.edu.

dagger Present address: Institut for Immunology, University of Vienna Medical School, A-1090 Vienna, Austria.

Journal of Virology, August 2001, p. 7175-7183, Vol. 75, No. 15
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.15.7175-7183.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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