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Journal of Virology, August 2001, p. 7142-7148, Vol. 75, No. 15
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.15.7142-7148.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Intrahepatic Genetic Inoculation of Hepatitis C Virus RNA Confers Cross-Protective Immunity

Amy J. Weiner,1,* Xavier Paliard,1 Mark J. Selby,1 Angelica Medina-Selby,1 Doris Coit,1 Steve Nguyen,1 Joe Kansopon,1 Christopher L. Arian,1 Philip Ng,1 Jeffery Tucker,1 Chun-Ting Lee,1 Noelle K. Polakos,1 Jang Han,1 Shirley Wong,1 Hui-Hua Lu,1 Steve Rosenberg,1 Kathy M. Brasky,2 David Chien,1 George Kuo,1 and Michael Houghton1

Chiron Corporation, Emeryville, California 94608,1 and Department of Laboratory Animal Medicine, Southwest Foundation for Biomedical Research, San Antonio, Texas 78227-53012

Received 1 February 2001/Accepted 25 April 2001

Naturally occurring hepatitis C virus (HCV) infection has long been thought to induce a weak immunity which is insufficient to protect an individual from subsequent infections and has cast doubt on the ability to develop effective vaccines. A series of intrahepatic genetic inoculations (IHGI) with type 1a HCV RNA were performed in a chimpanzee to determine whether a form of genetic immunization might stimulate protective immunity. We demonstrate that the chimpanzee not only developed protective immunity to the homologous type 1a RNA after rechallenge by IHGI but was also protected from chronic HCV infection after sequential rechallenge with 100 50% chimpanzee infectious doses of a heterologous type 1a (H77) and 1b (HC-J4) whole-virus inoculum. These results offer encouragement to pursue the development of HCV vaccines.

* Corresponding author. Mailing address: Chiron Corporation, LSC 4.3, 4560 Horton St., Emeryville, CA 94608. Phone: (510) 923-4785. Fax: (510) 923-2586. E-mail: amy_weiner{at}chiron.com.

Journal of Virology, August 2001, p. 7142-7148, Vol. 75, No. 15
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.15.7142-7148.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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