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Journal of Virology, June 2001, p. 5182-5188, Vol. 75, No. 11
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.11.5182-5188.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Identification of the Regions of Fv1 Necessary for Murine Leukemia Virus Restriction

Kate N. Bishop, Michael Bock, Greg Towers,dagger and Jonathan P. Stoye*

Division of Virology, National Institute for Medical Research, London NW7 1AA, United Kingdom

Received 10 January 2001/Accepted 14 March 2001

The Fv1 gene restricts murine leukemia virus replication via an interaction with the viral capsid protein. To study this interaction, a number of mutations, including a series of N-terminal and C-terminal deletions, internal deletions, and a number of single-amino-acid substitutions, were introduced into the n and b alleles of the Fv1 gene and the effects of these changes on virus restriction were measured. A significant fraction of the Fv1 protein was not required for restriction; however, retention of an intact major homology region as well as of domains toward the N and C termini was essential. Binding specificity appeared to be a combinatorial property of a number of residues within the C-terminal portion of Fv1.

* Corresponding author. Mailing address: Division of Virology, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, United Kingdom. Phone: 44-208/959-3666, ext. 2140. Fax: 44-208/906-4477. E-mail: jstoye{at}nimr.mrc.ac.uk.

dagger Present address: Wohl Virion Centre, Windeyer Institute of Medical Sciences, University College London, London W1P 6DB, United Kingdom.

Journal of Virology, June 2001, p. 5182-5188, Vol. 75, No. 11
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.11.5182-5188.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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