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Journal of Virology, June 2001, p. 5043-5048, Vol. 75, No. 11
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.11.5043-5048.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Incorporation of Lysyl-tRNA Synthetase into Human Immunodeficiency Virus Type 1

Shan Cen,1 Ahmad Khorchid,1,2 Hassan Javanbakht,1,2 Juliana Gabor,1,3 Timothy Stello,4 Kiyotaka Shiba,5 Karin Musier-Forsyth,4 and Lawrence Kleiman1,2,3,*

Lady Davis Institute for Medical Research and McGill AIDS Centre, Jewish General Hospital,1 and Departments of Medicine2 and Microbiology & Immunology,3 McGill University, Montreal, Quebec, Canada H3T 1E2; Department of Cell Biology, Cancer Institute, Japanese Foundation for Cancer Research, Kami-Ikebukuro, Toshima-ku, Tokyo 170, Japan5; and Department of Chemistry, University of Minnesota, Minneapolis, Minnesota 554554

Received 21 December 2000/Accepted 3 March 2001

During human immunodeficiency virus type 1 (HIV-1) assembly, tRNALys isoacceptors are selectively incorporated into virions and tRNA<UP><SUB>3</SUB><SUP>Lys</SUP></UP> is used as the primer for reverse transcription. We show herein that the tRNALys-binding protein, lysyl-tRNA synthetase (LysRS), is also selectively packaged into HIV-1. The viral precursor protein Pr55gag alone will package LysRS into Pr55gag particles, independently of tRNALys. With the additional presence of the viral precursor protein Pr160gag-pol, tRNALys and LysRS are both packaged into the particle. While the predominant cytoplasmic LysRS has an apparent Mr of 70,000, viral LysRS associated with tRNALys packaging is shorter, with an apparent Mr of 63,000. The truncation occurs independently of viral protease and might be required to facilitate interactions involved in the selective packaging and genomic placement of primer tRNA<UP><SUB>3</SUB><SUP>Lys</SUP></UP>.

* Corresponding author. Mailing address: Lady Davis Institute for Medical Research, Jewish General Hospital, 3755 Cote Ste-Catherine Road, Montreal, Quebec, Canada H3T 1E2. Phone: (514) 340-8260. Fax: (514) 340-7502. E-mail: lkleim{at}po-box.mcgill.ca.

Journal of Virology, June 2001, p. 5043-5048, Vol. 75, No. 11
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.11.5043-5048.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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