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Journal of Virology, May 2001, p. 4902-4906, Vol. 75, No. 10
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.10.4902-4906.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Identification of Critical Elements in the tRNA Acceptor Stem and TPsi C Loop Necessary for Human Immunodeficiency Virus Type 1 Infectivity

Qin Yu1 and Casey D. Morrow1,2,*

Departments of Microbiology1 and Cell Biology,2 University of Alabama at Birmingham, Birmingham, Alabama 35294

Received 14 November 2000/Accepted 19 February 2001

A mutant human immunodeficiency virus type 1 (HIV-1) with a primer binding site (PBS) complementary to yeast tRNAPhe (psHIV-Phe), which relies on exogenous yeast tRNAPhe as reverse transcription primer, was used to investigate elements in the tRNA acceptor stem and TPsi C stem-loop required for the tRNA primer selection and use in HIV-1 replication. tRNAPhe mutants with two- or four-nucleotide deletions in the 3' end retained the capacity to complement replication of psHIV-Phe. tRNAPhe mutants with an extended 5' end had reduced capacity for complementation, which could be restored by extension of the 3' end of these tRNAPhe mutants with sequences complementary to the HIV-1 U5 region. Further analysis of mutations in the acceptor stem of tRNAPhe suggested that an intact acceptor stem RNA structure is important for complementation. Analysis of single-nucleotide changes in the TPsi C stem-loop of tRNAPhe revealed an unexpected, essential role of this region for rescue of psHIV-Phe.

* Corresponding author. Mailing address: Departments of Microbiology and Cell Biology, University of Alabama at Birmingham, Birmingham, AL 35294. Phone: (205) 934-5705. Fax: (205) 934-1580. E-mail: casey_morrow{at}uab.edu.

Journal of Virology, May 2001, p. 4902-4906, Vol. 75, No. 10
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.10.4902-4906.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • McCulley, A., Morrow, C. D. (2006). Complementation of Human Immunodeficiency Virus Type 1 Replication by Intracellular Selection of Escherichia coli Formula Supplied in trans. J. Virol. 80: 9641-9650 [Abstract] [Full Text]  
  • Kelly, N. J., Morrow, C. D. (2005). Structural Elements of the tRNA T{Psi}C Loop Critical for Nucleocytoplasmic Transport Are Important for Human Immunodeficiency Virus Type 1 Primer Selection. J. Virol. 79: 6532-6539 [Abstract] [Full Text]  
  • Kelly, N. J., Palmer, M. T., Morrow, C. D. (2003). Selection of Retroviral Reverse Transcription Primer Is Coordinated with tRNA Biogenesis. J. Virol. 77: 8695-8701 [Abstract] [Full Text]  


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