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Journal of Virology, May 2001, p. 4490-4498, Vol. 75, No. 10
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.10.4490-4498.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Mus cervicolor Murine Leukemia Virus Isolate M813 Belongs to a Unique Receptor Interference Group

Vladimir Prassolov,^1,2 Sibyll Hein,² Marion Ziegler,² Dmitry Ivanov,¹ Carsten Münk,^2, Jürgen Löhler,³ and Carol Stocking^2,*

Engelhardt Institute of Molecular Biology, Moscow, Russia,¹ and Department of Cell and Virus Genetics² and the Group of Molecular Pathology,³ Heinrich Pette Institute for Experimental Immunology and Virology at Hamburg University, D-20251 Hamburg, Germany

Received 20 December 2000/Accepted 13 February 2001

Murine leukemia virus (MuLV) M813 was originally isolated from the Southeast Asian rodent Mus cervicolor. As with the ecotropic MuLVs derived from Mus musculus, its host range is limited to rodent cells. Earlier studies have mapped its receptor to chromosome 2, but it has not been established whether M813 shares a common receptor with any other MuLVs. In this study, we have performed interference assays with M813 and viruses from four interference groups of MuLV. The infection efficiency of M813 was not compromised in cells expressing any one of the other MuLVs, demonstrating that M813 must use a distinct receptor for cell entry. The entire M813 env coding region was molecularly cloned. Sequence analysis revealed high similarity with other MuLVs but with a unique receptor-binding domain. Substitution of M813 env sequences in Moloney MuLV resulted in a replication-competent virus with a host range and interference profile similar to those of the biological clone M813. M813 thus defines a novel receptor interference group of type C MuLVs.

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^* Corresponding author. Mailing address: Heinrich-Pette-Institut, Martinistr. 52, D-20251 Hamburg, Germany. Phone: 49 40 480 51 273. Fax: 49 40 480 51 187. E-mail: stocking{at}hpi.uni-hamburg.de.

Present address: The Salk Institute, Infectious Disease Laboratory, La Jolla, CA 92037.

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Journal of Virology, May 2001, p. 4490-4498, Vol. 75, No. 10
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.10.4490-4498.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

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