This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Thuman-Commike, P. A.
Right arrow Articles by Chiu, W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Thuman-Commike, P. A.
Right arrow Articles by Chiu, W.

 Previous Article  |  Next Article 

Journal of Virology, April 2000, p. 3871-3873, Vol. 74, No. 8
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Identification of Additional Coat-Scaffolding Interactions in a Bacteriophage P22 Mutant Defective in Maturation

Pamela A. Thuman-Commike,1,dagger Barrie Greene,2,Dagger Joanita Jakana,1 Amy McGough,1 Peter E. Prevelige,3 and Wah Chiu1,*

Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, Texas 770301; G. W. Hooper Foundation, University of California at San Francisco, San Francisco, California 941432; and Department of Microbiology, University of Alabama at Birmingham, Birmingham, Alabama 352943

Received 3 September 1999/Accepted 18 January 2000

Scaffolding proteins play a critical role in the assembly of certain viruses by directing the formation and maturation of a precursor capsid. Using electron cryomicroscopy difference mapping, we have identified an altered arrangement of a mutant scaffolding within the bacteriophage P22 procapsid. This mutant scaffolding allows us to directly visualize scaffolding density within the P22 procapsid. Based on these observations we propose a model for why the mutant prevents scaffolding release and capsid maturation.

* Corresponding author. Mailing address: Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030-3498. Phone: (713) 798-6985. Fax: (713) 798-1625. E-mail: wah{at}bcm.tmc.edu.

dagger Present address: QED Labs, Pleasanton, CA 94588.

Dagger Present address: Incyte Pharmaceuticals, Palo Alto, CA 94304.

Journal of Virology, April 2000, p. 3871-3873, Vol. 74, No. 8
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • McClelland, D. A., Aitken, J. D., Bhella, D., McNab, D., Mitchell, J., Kelly, S. M., Price, N. C., Rixon, F. J. (2002). pH Reduction as a Trigger for Dissociation of Herpes Simplex Virus Type 1 Scaffolds. J. Virol. 76: 7407-7417 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»