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Journal of Virology, April 2000, p. 3709-3714, Vol. 74, No. 8
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

A Novel Subgenomic Murine Leukemia Virus RNA Transcript Results from Alternative Splicing

Jérôme Déjardin,1 Guillaume Bompard-Maréchal,1 Muriel Audit,1 Thomas J. Hope,2 Marc Sitbon,1 and Marylène Mougel1,*

Institut de Génétique Moléculaire de Montpellier, IFR 24, CNRS-UMR5535, and Université Montpellier II, F-34293 Montpellier Cedex 5, France,1 and Salk Institute for Biological Studies, La Jolla, California 920372

Received 4 October 1999/Accepted 26 January 2000

Here we show the existence of a novel subgenomic 4.4-kb RNA in cells infected with the prototypic replication-competent Friend or Moloney murine leukemia viruses (MuLV). This RNA derives by splicing from an alternative donor site (SD') within the capsid-coding region to the canonical envelope splice acceptor site. The position and the sequence of SD' was highly conserved among mammalian type C and D oncoviruses. Point mutations used to inactivate SD' without changing the capsid-coding ability affected viral RNA splicing and reduced viral replication in infected cells.

* Corresponding author. Mailing address: Institut de Génétique Moléculaire de Montpellier II (IGMM), IFR 24, CNRS-UMR5535, Université Montpellier II, 1919, Rte. de Mende, F-34293 Montpellier Cedex 5, France. Phone: 33 (4) 67 61 36 40. Fax: 33 (4) 67 04 02 31. E-mail: mougel{at}igm.cnrs-mop.fr.

Journal of Virology, April 2000, p. 3709-3714, Vol. 74, No. 8
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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