This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Katano, H.
Right arrow Articles by Sata, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Katano, H.
Right arrow Articles by Sata, T.

 Previous Article  |  Next Article 

Journal of Virology, April 2000, p. 3478-3485, Vol. 74, No. 8
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Identification of Antigenic Proteins Encoded by Human Herpesvirus 8 and Seroprevalence in the General Population and among Patients with and without Kaposi's Sarcoma

Harutaka Katano,1 Takuya Iwasaki,1 Nobuyoshi Baba,1 Masanori Terai,1 Shigeo Mori,2 Aikichi Iwamoto,3 Takeshi Kurata,1 and Tetsutaro Sata1,4,*

Department of Pathology1 and Laboratory of Pathology,4 AIDS Research Center, National Institute of Infectious Diseases, Shinjuku, Tokyo 162-8640, and Departments of Pathology2 and Infectious Diseases,3 Institute of Medical Science, University of Tokyo, Minato, Tokyo 108-8639, Japan

Received 15 November 1999/Accepted 19 January 2000

To establish a sensitive and specific antibody assay, potent antigenic proteins encoded by human herpesvirus 8 (HHV8) were studied. Fifteen recombinant HHV8-encoded proteins were produced as glutathione S-transferase fusion proteins. The sera from AIDS-associated Kaposi's sarcoma (KS) patients reacted with four proteins encoded by open reading frames (ORFs) K8.1, 59, 65, and 73 in a Western blot assay. An enzyme-linked immunosorbent assay (ELISA) using these four proteins as antigens (mixed-antigen ELISA) revealed that all 26 sera derived from KS patients (24 with and 2 without human immunodeficiency virus infection) became positive for anti-HHV8 antibodies. The presence of HHV8 was demonstrated in 14 (1.4%) of 1,004 sera from the Japanese general population and 10 (1.9%) of 527 sera from patients without HHV8-associated diseases. The presence of immunoglobulin G (IgG) and IgM antibodies against HHV8 examined further by the mixed-antigen ELISA and Western blotting revealed IgG antibody in all ELISA-positive sera, while IgM antibody against ORF K8.1 was absent. These data suggest that the ORF 73 and 65 proteins are potent antigens for a sensitive serological assay.

* Corresponding author. Mailing address: Laboratory of Pathology, AIDS Research Center, and Department of Pathology, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku, Tokyo 162-8640, Japan. Phone: 81-3-5285-1111, ext. 2627. Fax: 81-3-5285-1189. E-mail: tsata{at}nih.go.jp.

Journal of Virology, April 2000, p. 3478-3485, Vol. 74, No. 8
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Burbelo, P. D., Leahy, H. P., Groot, S., Bishop, L. R., Miley, W., Iadarola, M. J., Whitby, D., Kovacs, J. A. (2009). Four-Antigen Mixture Containing V-Cyclin for Serological Screening of Human Herpesvirus 8 Infection. CVI 16: 621-627 [Abstract] [Full Text]  
  • Minhas, V., Crosby, L. N., Crabtree, K. L., Phiri, S., M'soka, T. J., Kankasa, C., Harrington, W. J., Mitchell, C. D., Wood, C. (2008). Development of an Immunofluorescence Assay Using Recombinant Proteins Expressed in Insect Cells To Screen and Confirm Presence of Human Herpesvirus 8-Specific Antibodies. CVI 15: 1259-1264 [Abstract] [Full Text]  
  • Verma, S. C., Choudhuri, T., Kaul, R., Robertson, E. S. (2006). Latency-Associated Nuclear Antigen (LANA) of Kaposi's Sarcoma-Associated Herpesvirus Interacts with Origin Recognition Complexes at the LANA Binding Sequence within the Terminal Repeats. J. Virol. 80: 2243-2256 [Abstract] [Full Text]  
  • Trento, E., Castilletti, C., Ferraro, C., Lesnoni La Parola, I., Mussi, A., Muscardin, L., Bordignon, V., D'Agosto, G., Amantea, A., Mastroianni, A., Ameglio, F., Fluhr, J., Cordiali-Fei, P. (2005). Human Herpesvirus 8 Infection in Patients With Cutaneous Lymphoproliferative Diseases. Arch Dermatol 141: 1235-1242 [Abstract] [Full Text]  
  • Ohsaki, E., Ueda, K., Sakakibara, S., Do, E., Yada, K., Yamanishi, K. (2004). Poly(ADP-Ribose) Polymerase 1 Binds to Kaposi's Sarcoma-Associated Herpesvirus (KSHV) Terminal Repeat Sequence and Modulates KSHV Replication in Latency. J. Virol. 78: 9936-9946 [Abstract] [Full Text]  
  • Kamiyama, K, Kinjo, T, Chinen, K, Iwamasa, T, Uezato, H, Miyagi, J-i, Mori, N, Yamane, N (2004). Human herpesvirus 8 (HHV8) sequence variations in HHV8 related tumours in Okinawa, a subtropical island in southern Japan. J. Clin. Pathol. 57: 529-535 [Abstract] [Full Text]  
  • Endo, T., Miura, T., Koibuchi, T., Nakamura, H., Takahashi, T., Odawara, T., Goto, M., Ajisawa, A., Iwamoto, A., Nakamura, T. (2003). Molecular Analysis of Human Herpesvirus 8 by Using Single Nucleotide Polymorphisms in Open Reading Frame 26. J. Clin. Microbiol. 41: 2492-2497 [Abstract] [Full Text]  
  • Sato, B., Ito, H., Hinchliffe, S., Sommer, M. H., Zerboni, L., Arvin, A. M. (2003). Mutational Analysis of Open Reading Frames 62 and 71, Encoding the Varicella-Zoster Virus Immediate-Early Transactivating Protein, IE62, and Effects on Replication In Vitro and in Skin Xenografts in the SCID-hu Mouse In Vivo. J. Virol. 77: 5607-5620 [Abstract] [Full Text]  
  • Coulter, L. J., Reid, H. W. (2002). Isolation and expression of three open reading frames from ovine herpesvirus-2. J. Gen. Virol. 83: 533-543 [Abstract] [Full Text]  
  • Lam, L. L., Pau, C.-P., Dollard, S. C., Pellett, P. E., Spira, T. J. (2002). Highly Sensitive Assay for Human Herpesvirus 8 Antibodies That Uses a Multiple Antigenic Peptide Derived from Open Reading Frame K8.1. J. Clin. Microbiol. 40: 325-329 [Abstract] [Full Text]  
  • Corchero, J. L., Mar, E.-C., Spira, T. J., Pellett, P. E., Inoue, N. (2001). Comparison of Serologic Assays for Detection of Antibodies against Human Herpesvirus 8. CVI 8: 913-921 [Abstract] [Full Text]  
  • Sakurada, S., Katano, H., Sata, T., Ohkuni, H., Watanabe, T., Mori, S. (2001). Effective Human Herpesvirus 8 Infection of Human Umbilical Vein Endothelial Cells by Cell-Mediated Transmission. J. Virol. 75: 7717-7722 [Abstract] [Full Text]  
  • Aoki, Y., Yarchoan, R., Wyvill, K., Okamoto, S.-i., Little, R. F., Tosato, G. (2001). Detection of viral interleukin-6 in Kaposi sarcoma-associated herpesvirus-linked disorders. Blood 97: 2173-2176 [Abstract] [Full Text]  
  • Meng, Y.-X., Sata, T., Stamey, F. R., Voevodin, A., Katano, H., Koizumi, H., Deleon, M., De Cristofano, M. A., Galimberti, R., Pellett, P. E. (2001). Molecular characterization of strains of Human herpesvirus 8 from Japan, Argentina and Kuwait. J. Gen. Virol. 82: 499-506 [Abstract] [Full Text]  
  • Davidovici, B., Karakis, I., Bourboulia, D., Ariad, S., Zong, J.-C., Benharroch, D., Dupin, N., Weiss, R., Hayward, G., Sarov, B., Boshoff, C. (2001). Seroepidemiology and Molecular Epidemiology of Kaposi's Sarcoma-Associated Herpesvirus Among Jewish Population Groups in Israel. JNCI J Natl Cancer Inst 93: 194-202 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»