This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Huang, M.
Right arrow Articles by Wong, S.-M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Huang, M.
Right arrow Articles by Wong, S.-M.

 Previous Article  |  Next Article 

Journal of Virology, April 2000, p. 3149-3155, Vol. 74, No. 7
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Complete Nucleotide Sequence and Genome Organization of Hibiscus Chlorotic Ringspot Virus, a New Member of the Genus Carmovirus: Evidence for the Presence and Expression of Two Novel Open Reading Frames

Mei Huang,1 Dora Chin-Yen Koh,2 Li-Juan Weng,2 Min-Li Chang,2 Yun-Kiam Yap,2 Lee Zhang,1 and Sek-Man Wong2,*

Institute of Molecular Agrobiology, Singapore 117604,1 and Department of Biological Sciences, The National University of Singapore, Singapore 117543,2 Republic of Singapore

Received 30 August 1999/Accepted 3 January 2000

The complete nucleotide sequence of hibiscus chlorotic ringspot virus (HCRSV) was determined. The genomic RNA (gRNA) is 3,911 nucleotides long and has the potential to encode seven viral proteins in the order of 28 (p28), 23 (p23), 81 (p81), 8 (p8), 9 (p9), 38 (p38), and 25 (p25) kDa. Excluding two unique open reading frames (ORFs) encoding p23 and p25, the ORFs encode proteins with high amino acid similarity to those of carmoviruses. In addition to gRNA, two 3'-coterminated subgenomic RNA (sgRNA) species were identified. Full-length cDNA clones derived from gRNA and sgRNA were constructed under the control of a T7 promoter. Both capped and uncapped transcripts derived from the full-length genomic cDNA clone were infectious. In vitro translation and mutagenesis assays confirmed that all the predicted ORFs except the ORF encoding p8 are translatable, and the two novel ORFs (those encoding p23 and p25) may be functionally indispensable for the viral infection cycle. Based on virion morphology and genome organization, we propose that HCRSV be classified as a new member of the genus Carmovirus in family Tombusviridae.

* Corresponding author. Mailing address: Department of Biological Sciences, The National University of Singapore, 10 Science Dr. 4, Singapore 117543, Republic of Singapore. Phone: 65-874-2976. Fax: 65-779-5671. E-mail: dbswsm{at}nus.edu.sg.

Journal of Virology, April 2000, p. 3149-3155, Vol. 74, No. 7
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Zhang, X., Wong, S.-M. (2009). Hibiscus chlorotic ringspot virus upregulates plant sulfite oxidase transcripts and increases sulfate levels in kenaf (Hibiscus cannabinus L.). J. Gen. Virol. 90: 3042-3050 [Abstract] [Full Text]  
  • Meng, C., Chen, J., Ding, S.-w., Peng, J., Wong, S.-M. (2008). Hibiscus chlorotic ringspot virus coat protein inhibits trans-acting small interfering RNA biogenesis in Arabidopsis. J. Gen. Virol. 89: 2349-2358 [Abstract] [Full Text]  
  • Ren, Y., Wong, S.-M., Lim, L.-Y. (2006). In vitro-reassembled plant virus-like particles for loading of polyacids. J. Gen. Virol. 87: 2749-2754 [Abstract] [Full Text]  
  • Genoves, A., Navarro, J. A., Pallas, V. (2006). Functional analysis of the five melon necrotic spot virus genome-encoded proteins.. J. Gen. Virol. 87: 2371-2380 [Abstract] [Full Text]  
  • Li, W., Wong, S.-M. (2006). Analyses of subgenomic promoters of hibiscus chlorotic ringspot virus and demonstration of 5' untranslated region and 3'-terminal sequences functioning as subgenomic promoters.. J. Virol. 80: 3395-3405 [Abstract] [Full Text]  
  • Meng, C., Chen, J., Peng, J., Wong, S.-M. (2006). Host-induced avirulence of hibiscus chlorotic ringspot virus mutants correlates with reduced gene-silencing suppression activity. J. Gen. Virol. 87: 451-459 [Abstract] [Full Text]  
  • Zhang, G., Zhang, J., Simon, A. E. (2004). Repression and Derepression of Minus-Strand Synthesis in a Plus-Strand RNA Virus Replicon. J. Virol. 78: 7619-7633 [Abstract] [Full Text]  
  • Wang, H.-H., Wong, S.-M. (2004). Significance of the 3'-terminal region in minus-strand RNA synthesis of Hibiscus chlorotic ringspot virus. J. Gen. Virol. 85: 1763-1776 [Abstract] [Full Text]  
  • Koh, D. C.-Y., Wong, S.-M., Liu, D. X. (2003). Synergism of the 3'-Untranslated Region and an Internal Ribosome Entry Site Differentially Enhances the Translation of a Plant Virus Coat Protein. J. Biol. Chem. 278: 20565-20573 [Abstract] [Full Text]  
  • Liang, X.-Z., Lucy, A. P., Ding, S.-W., Wong, S.-M. (2002). The p23 Protein of Hibiscus Chlorotic Ringspot Virus Is Indispensable for Host-Specific Replication. J. Virol. 76: 12312-12319 [Abstract] [Full Text]  
  • Liang, X.-Z., Lee, B. T. K., Wong, S.-M. (2002). Covariation in the Capsid Protein of Hibiscus Chlorotic Ringspot Virus Induced by Serial Passaging in a Host That Restricts Movement Leads to Avirulence in Its Systemic Host. J. Virol. 76: 12320-12324 [Abstract] [Full Text]  
  • Zamyatnin, A. A. Jr,, Solovyev, A. G., Sablina, A. A., Agranovsky, A. A., Katul, L., Vetten, H. J., Schiemann, J., Hinkkanen, A. E., Lehto, K., Morozov, S. Yu. (2002). Dual-colour imaging of membrane protein targeting directed by poa semilatent virus movement protein TGBp3 in plant and mammalian cells. J. Gen. Virol. 83: 651-662 [Abstract] [Full Text]  
  • Koh, D. C.-Y., Liu, D. X., Wong, S.-M. (2002). A Six-Nucleotide Segment within the 3' Untranslated Region of Hibiscus Chlorotic Ringspot Virus Plays an Essential Role in Translational Enhancement. J. Virol. 76: 1144-1153 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»