This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Masuda, M.
Right arrow Articles by Okayama, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Masuda, M.
Right arrow Articles by Okayama, H.

 Previous Article  |  Next Article 

Journal of Virology, March 2000, p. 2636-2646, Vol. 74, No. 6
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Genetic Studies with the Fission Yeast Schizosaccharomyces pombe Suggest Involvement of Wee1, Ppa2, and Rad24 in Induction of Cell Cycle Arrest by Human Immunodeficiency Virus Type 1 Vpr

Michiaki Masuda,1,* Yukiko Nagai,1 Norihito Oshima,1 Koichi Tanaka,2 Hiroshi Murakami,2 Hiroko Igarashi,1 and Hiroto Okayama2

Department of Microbiology1 and Department of Biochemistry and Molecular Biology,2 Graduate School of Medicine, The University of Tokyo, Tokyo 113-0033, Japan

Received 3 September 1999/Accepted 8 December 1999

Accessory protein Vpr of human immunodeficiency virus type 1 (HIV-1) arrests cell cycling at G2/M phase in human and simian cells. Recently, it has been shown that Vpr also causes cell cycle arrest in the fission yeast Schizosaccharomyces pombe, which shares the cell cycle regulatory mechanisms with higher eukaryotes including humans. In this study, in order to identify host cellular factors involved in Vpr-induced cell cycle arrest, the ability of Vpr to cause elongated cellular morphology (cdc phenotype) typical of G2/M cell cycle arrest in wild-type and various mutant strains of S. pombe was examined. Our results indicated that Vpr caused the cdc phenotype in wild-type S. pombe as well as in strains carrying mutations, such as the cdc2-3w, Delta cdc25, rad1-1, Delta chk1, Delta mik1, and Delta ppa1 strains. However, other mutants, such as the cdc2-1w, Delta wee1, Delta ppa2, and Delta rad24 strains, failed to show a distinct cdc phenotype in response to Vpr expression. Results of these genetic studies suggested that Wee1, Ppa2, and Rad24 might be required for induction of cell cycle arrest by HIV-1 Vpr. Cell proliferation was inhibited by Vpr expression in all of the strains examined including the ones that did not show the cdc phenotype. The results supported the previously suggested possibility that Vpr affects the cell cycle and cell proliferation through different pathways.

* Corresponding author. Mailing address: Department of Microbiology, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan. Phone: 81-3-5841-3409. Fax: 81-3-5841-3374. E-mail: mmasuda{at}m.u-tokyo.ac.jp.

Journal of Virology, March 2000, p. 2636-2646, Vol. 74, No. 6
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Kamata, M., Watanabe, N., Nagaoka, Y., Chen, I. S. Y. (2008). Human Immunodeficiency Virus Type 1 Vpr Binds to the N Lobe of the Wee1 Kinase Domain and Enhances Kinase Activity for Cdc2. J. Virol. 82: 5672-5682 [Abstract] [Full Text]  
  • Huard, S., Elder, R. T., Liang, D., Li, G., Zhao, R. Y. (2008). Human Immunodeficiency Virus Type 1 Vpr Induces Cell Cycle G2 Arrest through Srk1/MK2-Mediated Phosphorylation of Cdc25. J. Virol. 82: 2904-2917 [Abstract] [Full Text]  
  • Li, G., Elder, R. T., Qin, K., Park, H. U., Liang, D., Zhao, R. Y. (2007). Phosphatase Type 2A-dependent and -independent Pathways for ATR Phosphorylation of Chk1. J. Biol. Chem. 282: 7287-7298 [Abstract] [Full Text]  
  • Terada, Y., Yasuda, Y. (2006). Human Immunodeficiency Virus Type 1 Vpr Induces G2 Checkpoint Activation by Interacting with the Splicing Factor SAP145. Mol. Cell. Biol. 26: 8149-8158 [Abstract] [Full Text]  
  • Shimada, M., Namikawa-Yamada, C., Nakanishi, M., Murakami, H. (2005). Regulation of Cdc2p and Cdc13p Is Required for Cell Cycle Arrest Induced by Defective RNA Splicing in Fission Yeast. J. Biol. Chem. 280: 32640-32648 [Abstract] [Full Text]  
  • Kino, T., Gragerov, A., Valentin, A., Tsopanomihalou, M., Ilyina-Gragerova, G., Erwin-Cohen, R., Chrousos, G. P., Pavlakis, G. N. (2005). Vpr Protein of Human Immunodeficiency Virus Type 1 Binds to 14-3-3 Proteins and Facilitates Complex Formation with Cdc25C: Implications for Cell Cycle Arrest. J. Virol. 79: 2780-2787 [Abstract] [Full Text]  
  • Benko, Z., Liang, D., Agbottah, E., Hou, J., Chiu, K., Yu, M., Innis, S., Reed, P., Kabat, W., Elder, R. T., Di Marzio, P., Taricani, L., Ratner, L., Young, P. G., Bukrinsky, M., Zhao, R. Y. (2004). Anti-Vpr Activity of a Yeast Chaperone Protein. J. Virol. 78: 11016-11029 [Abstract] [Full Text]  
  • Iordanskiy, S., Zhao, Y., Dubrovsky, L., Iordanskaya, T., Chen, M., Liang, D., Bukrinsky, M. (2004). Heat Shock Protein 70 Protects Cells from Cell Cycle Arrest and Apoptosis Induced by Human Immunodeficiency Virus Type 1 Viral Protein R. J. Virol. 78: 9697-9704 [Abstract] [Full Text]  
  • Chang, F., Re, F., Sebastian, S., Sazer, S., Luban, J. (2004). HIV-1 Vpr Induces Defects in Mitosis, Cytokinesis, Nuclear Structure, and Centrosomes. Mol. Biol. Cell 15: 1793-1801 [Abstract] [Full Text]  
  • Planz, O., Pleschka, S., Oesterle, K., Berberich-Siebelt, F., Ehrhardt, C., Stitz, L., Ludwig, S. (2003). Borna Disease Virus Nucleoprotein Interacts with the Cdc2-Cyclin B1 Complex. J. Virol. 77: 11186-11192 [Abstract] [Full Text]  
  • Roshal, M., Kim, B., Zhu, Y., Nghiem, P., Planelles, V. (2003). Activation of the ATR-mediated DNA Damage Response by the HIV-1 Viral Protein R. J. Biol. Chem. 278: 25879-25886 [Abstract] [Full Text]  
  • Brasey, A., Lopez-Lastra, M., Ohlmann, T., Beerens, N., Berkhout, B., Darlix, J.-L., Sonenberg, N. (2003). The Leader of Human Immunodeficiency Virus Type 1 Genomic RNA Harbors an Internal Ribosome Entry Segment That Is Active during the G2/M Phase of the Cell Cycle. J. Virol. 77: 3939-3949 [Abstract] [Full Text]  
  • Yao, X.-J., Lemay, J., Rougeau, N., Clement, M., Kurtz, S., Belhumeur, P., Cohen, E. A. (2002). Genetic Selection of Peptide Inhibitors of Human Immunodeficiency Virus Type 1 Vpr. J. Biol. Chem. 277: 48816-48826 [Abstract] [Full Text]  
  • Kino, T., Gragerov, A., Slobodskaya, O., Tsopanomichalou, M., Chrousos, G. P., Pavlakis, G. N. (2002). Human Immunodeficiency Virus Type 1 (HIV-1) Accessory Protein Vpr Induces Transcription of the HIV-1 and Glucocorticoid-Responsive Promoters by Binding Directly to p300/CBP Coactivators. J. Virol. 76: 9724-9734 [Abstract] [Full Text]  
  • Davy, C. E., Jackson, D. J., Wang, Q., Raj, K., Masterson, P. J., Fenner, N. F., Southern, S., Cuthill, S., Millar, J. B. A., Doorbar, J. (2002). Identification of a G2 Arrest Domain in the E1{wedge}E4 Protein of Human Papillomavirus Type 16. J. Virol. 76: 9806-9818 [Abstract] [Full Text]  
  • Poggioli, G. J., DeBiasi, R. L., Bickel, R., Jotte, R., Spalding, A., Johnson, G. L., Tyler, K. L. (2002). Reovirus-Induced Alterations in Gene Expression Related to Cell Cycle Regulation. J. Virol. 76: 2585-2594 [Abstract] [Full Text]  
  • Poggioli, G. J., Dermody, T. S., Tyler, K. L. (2001). Reovirus-Induced {sigma}1s-Dependent G2/M Phase Cell Cycle Arrest Is Associated with Inhibition of p34cdc2. J. Virol. 75: 7429-7434 [Abstract] [Full Text]  
  • Wei, H., Ashby, D. G., Moreno, C. S., Ogris, E., Yeong, F. M., Corbett, A. H., Pallas, D. C. (2001). Carboxymethylation of the PP2A Catalytic Subunit in Saccharomyces cerevisiae Is Required for Efficient Interaction with the B-type Subunits Cdc55p and Rts1p. J. Biol. Chem. 276: 1570-1577 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»