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Journal of Virology, February 2000, p. 2038-2045, Vol. 74, No. 4
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Pathogenic Conversion of Live Attenuated Simian Immunodeficiency Virus Vaccines Is Associated with Expression of Truncated Nef

Earl T. Sawai,1,* M. Sabry Hamza,1 Michael Ye,1 Karen E. S. Shaw,2 and Paul A. Luciw1,2,*

Department of Medical Pathology1 and Center for Comparative Medicine,2 University of California, Davis, California 95616

Received 21 May 1999/Accepted 18 November 1999

Rhesus macaques infected with simian immunodeficiency virus (SIV) containing either a large nef deletion (SIVmac239Delta 152nef) or interleukin-2 in place of nef developed high virus loads and progressed to simian AIDS. Viruses recovered from both juvenile and neonatal macaques with disease produced a novel truncated Nef protein, tNef. Viruses recovered from juvenile macaques infected with serially passaged virus expressing tNef exhibited a pathogenic phenotype. These findings demonstrated strong selective pressure to restore expression of a truncated Nef protein, and this reversion was linked to increased pathogenic potential in live attenuated SIV vaccines.

* Corresponding author. Mailing address for Earl T. Sawai: Department of Medical Pathology, University of California, One Shields Ave., Davis, CA 95616. Phone: (530) 754-8873. Fax: (530) 752-4548. E-mail: etsawai{at}ucdavis.edu. Mailing address for Paul A. Luciw: Center for Comparative Medicine, University of California, Davis, One Shields Ave., Davis, CA 95616. Phone: (530) 752-3430. Fax: (530) 752-7914. E-mail: paluciw{at}ucdavis.edu.

Journal of Virology, February 2000, p. 2038-2045, Vol. 74, No. 4
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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