This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Vialat, P.
Right arrow Articles by Bouloy, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Vialat, P.
Right arrow Articles by Bouloy, M.

 Previous Article  |  Next Article 

Journal of Virology, February 2000, p. 1538-1543, Vol. 74, No. 3
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

The S Segment of Rift Valley Fever Phlebovirus (Bunyaviridae) Carries Determinants for Attenuation and Virulence in Mice

P. Vialat, A. Billecocq, A. Kohl, and M. Bouloy*

Groupe des Bunyaviridés, Institut Pasteur, 75724 Paris, Cedex 15, France

Received 10 August 1999/Accepted 21 October 1999

Unlike all the other Rift Valley fever virus strains (Bunyaviridae, Phlebovirus) studied so far, clone 13, a naturally attenuated virus, does not form the filaments composed of the NSs nonstructural protein in the nuclei of infected cells (R. Muller, J. F. Saluzzo, N. Lopez, T. Drier, M. Turell, J. Smith, and M. Bouloy, Am. J. Trop. Med. Hyg. 53:405-411, 1995). This defect is correlated with a large in-frame deletion in the NSs coding region of the S segment of the tripartite genome. Here, we show that the truncated NSs protein of clone 13 is expressed and remains in the cytoplasm, where it is degraded rapidly by the proteasome. Through the analysis of reassortants between clone 13 and a virulent strain, we localized the marker(s) of attenuation in the S segment of this attenuated virus. This result raises questions regarding the role of NSs in pathogenesis and highlights, for the first time in the Bunyaviridae family, a major role of the S segment in virulence and attenuation, possibly associated with a defect in the nonstructural protein.

* Corresponding author. Mailing address: Groupe des Bunyaviridés, Institut Pasteur, 25 rue du Dr. Roux, 75724 Paris, Cedex 15, France. Phone: 33 1 40 61 31 57. Fax: 33 1 40 61 31 51. E-mail: mbouloy{at}pasteur.fr.

Journal of Virology, February 2000, p. 1538-1543, Vol. 74, No. 3
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Habjan, M., Pichlmair, A., Elliott, R. M., Overby, A. K., Glatter, T., Gstaiger, M., Superti-Furga, G., Unger, H., Weber, F. (2009). NSs Protein of Rift Valley Fever Virus Induces the Specific Degradation of the Double-Stranded RNA-Dependent Protein Kinase. J. Virol. 83: 4365-4375 [Abstract] [Full Text]  
  • Habjan, M., Penski, N., Spiegel, M., Weber, F. (2008). T7 RNA polymerase-dependent and -independent systems for cDNA-based rescue of Rift Valley fever virus. J. Gen. Virol. 89: 2157-2166 [Abstract] [Full Text]  
  • Bird, B. H., Albarino, C. G., Hartman, A. L., Erickson, B. R., Ksiazek, T. G., Nichol, S. T. (2008). Rift Valley Fever Virus Lacking the NSs and NSm Genes Is Highly Attenuated, Confers Protective Immunity from Virulent Virus Challenge, and Allows for Differential Identification of Infected and Vaccinated Animals. J. Virol. 82: 2681-2691 [Abstract] [Full Text]  
  • Xu, F., Chen, H., Travassos da Rosa, A. P. A., Tesh, R. B., Xiao, S.-Y. (2007). Phylogenetic relationships among sandfly fever group viruses (Phlebovirus: Bunyaviridae) based on the small genome segment. J. Gen. Virol. 88: 2312-2319 [Abstract] [Full Text]  
  • XU, F., LIU, D., NUNES, M. R. T., DA ROSA, A. P. A. T., TESH, R. B., XIAO, S.-Y. (2007). ANTIGENIC AND GENETIC RELATIONSHIPS AMONG RIFT VALLEY FEVER VIRUS AND OTHER SELECTED MEMBERS OF THE GENUS PHLEBOVIRUS (BUNYAVIRIDAE). Am J Trop Med Hyg 76: 1194-1200 [Abstract] [Full Text]  
  • Perrone, L. A., Narayanan, K., Worthy, M., Peters, C. J. (2007). The S Segment of Punta Toro Virus (Bunyaviridae, Phlebovirus) Is a Major Determinant of Lethality in the Syrian Hamster and Codes for a Type I Interferon Antagonist. J. Virol. 81: 884-892 [Abstract] [Full Text]  
  • Billecocq, A., Spiegel, M., Vialat, P., Kohl, A., Weber, F., Bouloy, M., Haller, O. (2004). NSs Protein of Rift Valley Fever Virus Blocks Interferon Production by Inhibiting Host Gene Transcription. J. Virol. 78: 9798-9806 [Abstract] [Full Text]  
  • Kohl, A., Clayton, R. F., Weber, F., Bridgen, A., Randall, R. E., Elliott, R. M. (2003). Bunyamwera Virus Nonstructural Protein NSs Counteracts Interferon Regulatory Factor 3-Mediated Induction of Early Cell Death. J. Virol. 77: 7999-8008 [Abstract] [Full Text]  
  • Bucher, E., Sijen, T., de Haan, P., Goldbach, R., Prins, M. (2002). Negative-Strand Tospoviruses and Tenuiviruses Carry a Gene for a Suppressor of Gene Silencing at Analogous Genomic Positions. J. Virol. 77: 1329-1336 [Abstract] [Full Text]  
  • Flick, R., Pettersson, R. F. (2001). Reverse Genetics System for Uukuniemi Virus (Bunyaviridae): RNA Polymerase I-Catalyzed Expression of Chimeric Viral RNAs. J. Virol. 75: 1643-1655 [Abstract] [Full Text]  
  • Bouloy, M., Janzen, C., Vialat, P., Khun, H., Pavlovic, J., Huerre, M., Haller, O. (2001). Genetic Evidence for an Interferon-Antagonistic Function of Rift Valley Fever Virus Nonstructural Protein NSs. J. Virol. 75: 1371-1377 [Abstract] [Full Text]  
  • Ebihara, H., Yoshimatsu, K., Ogino, M., Araki, K., Ami, Y., Kariwa, H., Takashima, I., Li, D., Arikawa, J. (2000). Pathogenicity of Hantaan Virus in Newborn Mice: Genetic Reassortant Study Demonstrating that a Single Amino Acid Change in Glycoprotein G1 Is Related to Virulence. J. Virol. 74: 9245-9255 [Abstract] [Full Text]  
  • Billecocq, A., Vazeille-Falcoz, M., Rodhain, F., Bouloy, M. (2000). Pathogen-specific resistance to Rift Valley fever virus infection is induced in mosquito cells by expression of the recombinant nucleoprotein but not NSs non-structural protein sequences. J. Gen. Virol. 81: 2161-2166 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»