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Journal of Virology, February 2000, p. 1533-1537, Vol. 74, No. 3
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Cloning the Antibody Response in Humans with Chronic Inflammatory Disease: Immunopanning of Subacute Sclerosing Panencephalitis (SSPE) Brain Sections with Antibody Phage Libraries Prepared from SSPE Brain Enriches for Antibody Recognizing Measles Virus Antigens In Situ

Gregory P. Owens,1 R. Anthony Williamson,2 Mark P. Burgoon,1 Omar Ghausi,2 Dennis R. Burton,2 and Donald H. Gilden1,3,*

Departments of Neurology1 and Microbiology,3 University of Colorado Health Sciences Center, Denver, Colorado 80262, and Department of Immunology, Scripps Research Institute, La Jolla, California 920372

Received 23 July 1999/Accepted 20 October 1999

In central nervous system (CNS) infectious and inflammatory diseases of known cause, oligoclonal bands represent antibody directed against the causative agent. To determine whether disease-relevant antibodies can be cloned from diseased brain, we prepared an antibody phage display library from the brain of a human with subacute sclerosing panencephalitis (SSPE), a chronic encephalitis caused by measles virus, and selected the library against SSPE brain sections. Antibodies that were retrieved reacted strongly with measles virus cell extracts by enzyme-linked immunosorbent assay and were specific for the measles virus nucleocapsid protein. These antibodies immunostained cells in different SSPE brains but not in control brain. Our data provide the first demonstration that diseased brain can be used to select in situ for antibodies directed against the causative agent of disease and point to the potential usefulness of this approach in identifying relevant antibodies in chronic CNS or systemic inflammatory diseases of unknown cause.

* Corresponding author. Mailing address: Department of Neurology, Mailstop B182, University of Colorado Health Sciences Center, 4200 E. 9th Ave., Denver, CO 80262. Phone: (303) 315-8281. Fax: (303) 315-8720. E-mail: don.gilden{at}uchsc.edu.

Journal of Virology, February 2000, p. 1533-1537, Vol. 74, No. 3
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

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  • Gilden, D. H., Burgoon, M. P., Kleinschmidt-DeMasters, B. K., Williamson, R. A., Ghausi, O., Burton, D. R., Owens, G. P. (2001). Molecular Immunologic Strategies to Identify Antigens and B-Cell Responses Unique to Multiple Sclerosis. Arch Neurol 58: 43-48 [Abstract] [Full Text]  


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