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Journal of Virology, December 2000, p. 11950-11954, Vol. 74, No. 24
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Binding of Rabbit Hemorrhagic Disease Virus to Antigens of the ABH Histo-Blood Group Family

Nathalie Ruvoën-Clouet,1 Jean Pierre Ganière,1 Geneviève André-Fontaine,1 Dominique Blanchard,2 and Jacques Le Pendu3,*

Unité de Pathologie Infectieuse, Ecole Nationale Vétérinaire, 44307 Nantes cedex 03,1 Etablissement de Transfusion Sanguine, Pays de la Loire, 44011 Nantes cedex 01,2 and INSERM U419, Institut de Biologie, 44093 Nantes,3 France

Received 21 March 2000/Accepted 14 September 2000

The ability of rabbit hemorrhagic disease virus to agglutinate human erythrocytes and to attach to rabbit epithelial cells of the upper respiratory and digestive tracts was shown to depend on the presence of ABH blood group antigens. Indeed, agglutination was inhibited by saliva from secretor individuals but not from nonsecretors, the latter being devoid of H antigen. In addition, erythrocytes of the rare Bombay phenotype, which completely lack ABH antigens, were not agglutinated. Native viral particles from extracts of infected rabbit liver as well as virus-like particles from the recombinant virus capsid protein specifically bound to synthetic A and H type 2 blood group oligosaccharides. Both types of particles could attach to adult rabbit epithelial cells of the upper respiratory and digestive tracts. This binding paralleled that of anti-H type 2 blood group reagents and was inhibited by the H type 2-specific lectin UEA-I and polyacrylamide-conjugated H type 2 trisaccharide. Young rabbit tissues were almost devoid of A and H type 2 antigens, and only very weak binding of virus particles could be obtained on these tissues.

* Corresponding author. Mailing address: INSERM U419, Institut de Biologie, 9 quai Moncousu, 44093 Nantes, France. Phone: 33 240 08 40 99. Fax: 33 240 08 40 82. E-mail: jlependu{at}nantes.inserm.fr.

Journal of Virology, December 2000, p. 11950-11954, Vol. 74, No. 24
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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