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Journal of Virology, December 2000, p. 10873-10881, Vol. 74, No. 23
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

The ORF1 Products of Tombusviruses Play a Crucial Role in Lethal Necrosis of Virus-Infected Plants

József Burgyán,1,* Csaba Hornyik,1 György Szittya,1 Dániel Silhavy,1 and György Bisztray2

Agricultural Biotechnology Center, 2101 Gödöllö,1 and Department of Genetics and Horticultural Plant Breeding, Szent István University, Budapest,2 Hungary

Received 17 May 2000/Accepted 16 August 2000

Hybrids of cymbidium ringspot (CymRSV) and carnation Italian ringspot (CIRV) tombusviruses were used to identify viral symptom determinants responsible for the generalized necrosis in tombusvirus-infected plants. Surprisingly, symptoms of Nicotiana benthamiana infected with CymRSV/CIRV hybrids were distinctly different. It was demonstrated that not all chimeras expressing wild-type (wt) levels of p19 protein caused systemic necrosis as both parents CymRSV and CIRV did. We showed here that hybrids containing chimeric ORF1 were not able to induce lethal necrosis even if the viral replication of these constructs was not altered significantly. However, if a wt p33 (product of ORF1) of CymRSV was provided in trans in transgenic plants expressing p33 and its readthrough product p92, the lethal necrosis characteristic to tombusvirus infection was restored. In addition, the expression of p33 by a potato virus X viral vector in N. benthamiana caused severe chlorosis and occasionally necrosis, indicating the importance of p33 in wt symptoms of tombusviruses. Thus, our results provide evidence that elicitation of the necrotic phenotype requires the presence of the wt p33 in addition to the p19 protein of tombusviruses.

* Corresponding author. Mailing address: Agricultural Biotechnology Center, P.O. Box 411, 2101 Gödöllö, Hungary. Phone: (36-28)430 600. Fax: (36-28)430 482. E-mail: burgyan{at}abc.hu.

Journal of Virology, December 2000, p. 10873-10881, Vol. 74, No. 23
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

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