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Journal of Virology, November 2000, p. 10801-10806, Vol. 74, No. 22
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Rotavirus Infection Induces Cytoskeleton Disorganization in Human Intestinal Epithelial Cells: Implication of an Increase in Intracellular Calcium Concentration

Jean-Philippe Brunet,* Nathalie Jourdan, Jacqueline Cotte-Laffitte, Catherine Linxe, Monique Géniteau-Legendre, Alain Servin, and Anne-Marie Quéro

Institut National de la Santé et de la Recherche Médicale, Unité 510, Pathogènes et Fonctions des Cellules Épithéliales Polarisées, Faculté de Pharmacie, Université Paris XI, 92296 Châtenay-Malabry cedex, France

Received 25 May 2000/Accepted 23 August 2000

Rotavirus infection is the most common cause of severe infantile gastroenteritis worldwide. In vivo, rotavirus exhibits a marked tropism for the differentiated enterocytes of the intestinal epithelium. In vitro, differentiated and undifferentiated intestinal cells can be infected. We observed that rotavirus infection of the human intestinal epithelial Caco-2 cells induces cytoskeleton alterations as a function of cell differentiation. The vimentin network disorganization detected in undifferentiated Caco-2 cells was not found in fully differentiated cells. In contrast, differentiated Caco-2 cells presented Ca2+-dependent microtubule disassembly and Ca2+-independent cytokeratin 18 rearrangement, which both require viral replication. We propose that these structural alterations could represent the first manifestations of rotavirus-infected enterocyte injury leading to functional perturbations and then to diarrhea.

* Corresponding author. Mailing address: INSERM U-510, Faculté de Pharmacie, 5 rue J. B. Clément, 92296 Châtenay-Malabry cedex, France. Phone: 33-1 46 83 55 24. Fax: 33-1 46 83 58 83. E-mail: jean-philippe.brunet{at}cep.u-psud.fr.

Journal of Virology, November 2000, p. 10801-10806, Vol. 74, No. 22
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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