This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Balasuriya, U. B. R.
Right arrow Articles by MacLachlan, N. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Balasuriya, U. B. R.
Right arrow Articles by MacLachlan, N. J.

 Previous Article  |  Next Article 

Journal of Virology, November 2000, p. 10623-10630, Vol. 74, No. 22
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Expression of the Two Major Envelope Proteins of Equine Arteritis Virus as a Heterodimer Is Necessary for Induction of Neutralizing Antibodies in Mice Immunized with Recombinant Venezuelan Equine Encephalitis Virus Replicon Particles

Udeni B. R. Balasuriya,1 Hans W. Heidner,2 Jodi F. Hedges,1 Jacqueline C. Williams,2 Nancy L. Davis,3 Robert E. Johnston,3 and N. James MacLachlan1,*

Bernard and Gloria Salick Equine Viral Disease Laboratory, Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis, California 956161; Division of Life Sciences, University of Texas at San Antonio, San Antonio, Texas 782492; and Department of Microbiology and Immunology, University of North Carolina, Chapel Hill, North Carolina 275993

Received 16 May 2000/Accepted 15 August 2000

RNA replicon particles derived from a vaccine strain of Venezuelan equine encephalitis virus (VEE) were used as a vector for expression of the major envelope proteins (GL and M) of equine arteritis virus (EAV), both individually and in heterodimer form (GL/M). Open reading frame 5 (ORF5) encodes the GL protein, which expresses the known neutralizing determinants of EAV (U. B. R. Balasuriya, J. F. Patton, P. V. Rossitto, P. J. Timoney, W. H. McCollum, and N. J. MacLachlan, Virology 232:114-128, 1997). ORF5 and ORF6 (which encodes the M protein) of EAV were cloned into two different VEE replicon vectors that contained either one or two 26S subgenomic mRNA promoters. These replicon RNAs were packaged into VEE replicon particles by VEE capsid protein and glycoproteins supplied in trans in cells that were coelectroporated with replicon and helper RNAs. The immunogenicity of individual replicon particle preparations (pVR21-GL, pVR21-M, and pVR100-GL/M) in BALB/c mice was determined. All mice developed antibodies against the recombinant proteins with which they were immunized, but only the mice inoculated with replicon particles expressing the GL/M heterodimer developed antibodies that neutralize EAV. The data further confirmed that authentic posttranslational modification and conformational maturation of the recombinant GL protein occur only in the presence of the M protein and that this interaction is necessary for induction of neutralizing antibodies.

* Corresponding author. Mailing address: Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, One Shields Ave., University of California, Davis, CA 95616. Phone: (530) 752-1385. Fax: (530) 754-8124. E-mail: njmaclachlan{at}ucdavis.edu.

Journal of Virology, November 2000, p. 10623-10630, Vol. 74, No. 22
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Frieman, M., Ratia, K., Johnston, R. E., Mesecar, A. D., Baric, R. S. (2009). Severe Acute Respiratory Syndrome Coronavirus Papain-Like Protease Ubiquitin-Like Domain and Catalytic Domain Regulate Antagonism of IRF3 and NF-{kappa}B Signaling. J. Virol. 83: 6689-6705 [Abstract] [Full Text]  
  • Go, Y. Y., Wong, S. J., Branscum, A. J., Demarest, V. L., Shuck, K. M., Vickers, M. L., Zhang, J., McCollum, W. H., Timoney, P. J., Balasuriya, U. B. R. (2008). Development of a Fluorescent-Microsphere Immunoassay for Detection of Antibodies Specific to Equine Arteritis Virus and Comparison with the Virus Neutralization Test. CVI 15: 76-87 [Abstract] [Full Text]  
  • Reap, E. A., Dryga, S. A., Morris, J., Rivers, B., Norberg, P. K., Olmsted, R. A., Chulay, J. D. (2007). Cellular and Humoral Immune Responses to Alphavirus Replicon Vaccines Expressing Cytomegalovirus pp65, IE1, and gB Proteins. CVI 14: 748-755 [Abstract] [Full Text]  
  • Thomas, C. E., Zhu, W., Van Dam, C. N., Davis, N. L., Johnston, R. E., Sparling, P. F. (2006). Vaccination of Mice with Gonococcal TbpB Expressed In Vivo from Venezuelan Equine Encephalitis Viral Replicon Particles. Infect. Immun. 74: 1612-1620 [Abstract] [Full Text]  
  • Zhu, W., Thomas, C. E., Chen, C.-j., Van Dam, C. N., Johnston, R. E., Davis, N. L., Sparling, P. F. (2005). Comparison of Immune Responses to Gonococcal PorB Delivered as Outer Membrane Vesicles, Recombinant Protein, or Venezuelan Equine Encephalitis Virus Replicon Particles. Infect. Immun. 73: 7558-7568 [Abstract] [Full Text]  
  • Gehrke, R., Heinz, F. X., Davis, N. L., Mandl, C. W. (2005). Heterologous gene expression by infectious and replicon vectors derived from tick-borne encephalitis virus and direct comparison of this flavivirus system with an alphavirus replicon. J. Gen. Virol. 86: 1045-1053 [Abstract] [Full Text]  
  • Balasuriya, U. B. R., Hedges, J. F., Smalley, V. L., Navarrette, A., McCollum, W. H., Timoney, P. J., Snijder, E. J., MacLachlan, N. J. (2004). Genetic characterization of equine arteritis virus during persistent infection of stallions. J. Gen. Virol. 85: 379-390 [Abstract] [Full Text]  
  • Castillo-Olivares, J., Wieringa, R., Bakonyi, T., de Vries, A. A. F., Davis-Poynter, N. J., Rottier, P. J. M. (2003). Generation of a Candidate Live Marker Vaccine for Equine Arteritis Virus by Deletion of the Major Virus Neutralization Domain. J. Virol. 77: 8470-8480 [Abstract] [Full Text]  
  • Thomas, J. M., Klimstra, W. B., Ryman, K. D., Heidner, H. W. (2003). Sindbis Virus Vectors Designed To Express a Foreign Protein as a Cleavable Component of the Viral Structural Polyprotein. J. Virol. 77: 5598-5606 [Abstract] [Full Text]  
  • Curtis, K. M., Yount, B., Baric, R. S. (2002). Heterologous Gene Expression from Transmissible Gastroenteritis Virus Replicon Particles. J. Virol. 76: 1422-1434 [Abstract] [Full Text]  
  • Castillo-Olivares, J., de Vries, A. A. F., Raamsman, M. J. B., Rottier, P. J. M., Lakhani, K., Westcott, D., Tearle, J. P., Wood, J. L. N., Mumford, J. A., Hannant, D., Davis-Poynter, N. J. (2001). Evaluation of a prototype sub-unit vaccine against equine arteritis virus comprising the entire ectodomain of the virus large envelope glycoprotein (GL): induction of virus-neutralizing antibody and assessment of protection in ponies. J. Gen. Virol. 82: 2425-2435 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»