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Journal of Virology, November 2000, p. 9889-9894, Vol. 74, No. 21
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Efficient Site-Specific Nonribozyme Opening of Hepatitis Delta Virus Genomic RNA in Infected Livers

Jinhong Chang, Gloria Moraleda, Severin Gudima, and John Taylor*

Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111-2497

Received 19 May 2000/Accepted 10 August 2000

Examination of the 1,679-nucleotide (nt) unit-length hepatitis delta virus (HDV) RNAs in the livers of two HDV-infected woodchucks showed that 96% of the antigenomic RNA but only 50% of the genomic RNA was circular. We subsequently found that at least half of the linear unit-length genomic RNA was open at a unique location. Using a modified form of RNA ligation-mediated amplification of cDNA ends, we showed that the 5' end was located at nt 1212. Like the previously described ribozyme cleavage site at nt 686, the new site produced a 5'-OH. Nevertheless, we showed that this novel site was not produced by activity of the HDV genomic ribozyme. We speculate that the 5' end at nt 1212 reflects a preferred site of posttranscriptional endonucleolytic cleavage of genomic RNA.

* Corresponding author. Mailing address: Fox Chase Cancer Center, 7701 Burholme Ave., Philadelphia, PA 19111-2497. Phone: (215) 728-2436. Fax: (215) 728-3105. E-mail: JM_Taylor{at}FCCC.edu.

Journal of Virology, November 2000, p. 9889-9894, Vol. 74, No. 21
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Gudima, S. O., Chang, J., Taylor, J. M. (2004). Features Affecting the Ability of Hepatitis Delta Virus RNAs To Initiate RNA-Directed RNA Synthesis. J. Virol. 78: 5737-5744 [Abstract] [Full Text]  
  • Nie, X., Chang, J., Taylor, J. M. (2004). Alternative Processing of Hepatitis Delta Virus Antigenomic RNA Transcripts. J. Virol. 78: 4517-4524 [Abstract] [Full Text]  
  • Chang, J., Taylor, J. M. (2003). Susceptibility of Human Hepatitis Delta Virus RNAs to Small Interfering RNA Action. J. Virol. 77: 9728-9731 [Abstract] [Full Text]  
  • Chang, J., Sigal, L. J., Lerro, A., Taylor, J. (2001). Replication of the Human Hepatitis Delta Virus Genome Is Initiated in Mouse Hepatocytes following Intravenous Injection of Naked DNA or RNA Sequences. J. Virol. 75: 3469-3473 [Abstract] [Full Text]  


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