This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Chen, J.
Right arrow Articles by Stinski, M. F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chen, J.
Right arrow Articles by Stinski, M. F.

 Previous Article  |  Next Article 

Journal of Virology, November 2000, p. 9845-9857, Vol. 74, No. 21
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Activation of Transcription of the Human Cytomegalovirus Early UL4 Promoter by the Ets Transcription Factor Binding Element

Jiping Chen and Mark F. Stinski*

Department of Microbiology, College of Medicine, University of Iowa, Iowa City, Iowa 52242

Received 17 April 2000/Accepted 28 July 2000

The human cytomegalovirus (HCMV) early UL4 promoter has served as a useful model for studying the activation of early viral gene expression. Previous transient-transfection experiments detected cis-acting elements (the NF-Y site and site 2) upstream of the transcriptional start site (L. Huang and M. F. Stinski, J. Virol. 69:7612-7621, 1995). The roles of two of these sites, the NF-Y site and site 2, in the context of the viral genome were investigated further by comparing mRNA levels from the early UL4 promoter in human foreskin fibroblasts infected by recombinant viruses with either wild-type or mutant cis-acting elements. Steady-state mRNA levels from the UL4 promoter with a mutation in the NF-Y site were comparable to that of wild type. A mutation in an Elk-1 site plus putative IE86 protein binding sites decreased the steady-state mRNA levels compared to the wild type at early times after infection. Electrophoretic mobility shift assays and antibody supershifts detected the binding of cellular transcription factor Elk-1 to site 2 DNA with infected nuclear extracts but not with mock-infected nuclear extracts. The role of cellular transcription factors activated by the mitogen activated protein kinase/extracellular signal-regulated kinase pathway in activating transcription from early viral promoters is discussed.

* Corresponding author. Mailing address: Department of Microbiology, College of Medicine, University of Iowa, Iowa City, Iowa 52242. Phone: (319) 335-7792. Fax: (319) 335-9006. E-mail: mark-stinski{at}uiowa.edu.

Journal of Virology, November 2000, p. 9845-9857, Vol. 74, No. 21
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Hannemann, H., Rosenke, K., O'Dowd, J. M., Fortunato, E. A. (2009). The Presence of p53 Influences the Expression of Multiple Human Cytomegalovirus Genes at Early Times Postinfection. J. Virol. 83: 4316-4325 [Abstract] [Full Text]  
  • Chia, M. C., Leung, A., Krushel, T., Alajez, N. M., Lo, K. W., Busson, P., Klamut, H. J., Bastianutto, C., Liu, F.-F. (2008). Nuclear Factor-Y and Epstein Barr Virus in Nasopharyngeal Cancer. Clin. Cancer Res. 14: 984-994 [Abstract] [Full Text]  
  • Petrik, D. T., Schmitt, K. P., Stinski, M. F. (2007). The Autoregulatory and Transactivating Functions of the Human Cytomegalovirus IE86 Protein Use Independent Mechanisms for Promoter Binding. J. Virol. 81: 5807-5818 [Abstract] [Full Text]  
  • Lyman, M. G., Randall, J. A., Calton, C. M., Banfield, B. W. (2006). Localization of ERK/MAP Kinase Is Regulated by the Alphaherpesvirus Tegument Protein Us2. J. Virol. 80: 7159-7168 [Abstract] [Full Text]  
  • Gribaudo, G., Riera, L., Rudge, T. L., Caposio, P., Johnson, L. F., Landolfo, S. (2002). Human cytomegalovirus infection induces cellular thymidylate synthase gene expression in quiescent fibroblasts. J. Gen. Virol. 83: 2983-2993 [Abstract] [Full Text]  
  • Chen, J., Stinski, M. F. (2002). Role of Regulatory Elements and the MAPK/ERK or p38 MAPK Pathways for Activation of Human Cytomegalovirus Gene Expression. J. Virol. 76: 4873-4885 [Abstract] [Full Text]  
  • Spaderna, S., Blessing, H., Bogner, E., Britt, W., Mach, M. (2002). Identification of Glycoprotein gpTRL10 as a Structural Component of Human Cytomegalovirus. J. Virol. 76: 1450-1460 [Abstract] [Full Text]  
  • Heider, J. A., Yu, Y., Shenk, T., Alwine, J. C. (2002). Characterization of a Human Cytomegalovirus with Phosphorylation Site Mutations in the Immediate-Early 2 Protein. J. Virol. 76: 928-932 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»