This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Cabot, B.
Right arrow Articles by Gómez, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cabot, B.
Right arrow Articles by Gómez, J.

 Previous Article  |  Next Article 

Journal of Virology, January 2000, p. 805-811, Vol. 74, No. 2
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Nucleotide and Amino Acid Complexity of Hepatitis C Virus Quasispecies in Serum and Liver

Beatriz Cabot, María Martell, Juan I. Esteban,* Sílvia Sauleda, Teresa Otero, Rafael Esteban, Jaime Guàrdia, and Jordi Gómez

Liver Unit, Department of Internal Medicine, Hospital General Universitari Vall d'Hebron, Universitat Autònoma de Barcelona, 08035 Barcelona, Spain

Received 15 July 1999/Accepted 19 October 1999

The quasispecies nature of the hepatitis C virus (HCV) is thought to play a central role in maintaining and modulating viral replication. Several studies have tried to unravel, through the parameters that characterize HCV circulating quasispecies, prognostic markers of the disease. In a previous work we demonstrated that the parameters of circulating viral quasispecies do not always reflect those of the intrahepatic virus. Here, we have analyzed paired serum and liver quasispecies from 39 genotype 1b-infected patients with different degrees of liver damage, ranging from minimal changes to cirrhosis. Viral level was quantified by real-time reverse transcription-PCR, and viral heterogeneity was characterized through the cloning and sequencing of 540 HCV variants of a genomic fragment encompassing the E2-NS2 junction. Although in 95% of patients, serum and liver consensus HCV amino acid sequences were identical, quasispecies complexity varied considerably between the viruses isolated from each compartment. Patients with HCV quasispecies in serum more complex (26%) than, less complex (28%) than, or similarly complex (41%) to those in liver were found. Among the last, a significant correlation between fibrosis and all the parameters that measure the viral amino acid complexity was found. Correlation between fibrosis and serum viral load was found as well (R = 0.7). With regard to the origin of the differences in quasispecies complexity between serum and liver populations, sequence analysis argued against extrahepatic replication as a quantitatively important contributing factor and supported the idea of a differential effect or different selective forces on the virus depending on whether it is circulating in serum or replicating in the liver.

* Corresponding author. Mailing address: Laboratori de Medicina Interna-Hepatologia, Unitat B d'Investigació (Edifici de Magatzems Generals), Hospital Vall d'Hebron, Passeig Vall d'Hebron 119, 08035 Barcelona, Spain. Phone: 34-93 4894934. Fax: 34-93 4894032. E-mail: jgomez{at}hg.vhebron.es.

Journal of Virology, January 2000, p. 805-811, Vol. 74, No. 2
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Howe, A. Y. M., Cheng, H., Johann, S., Mullen, S., Chunduru, S. K., Young, D. C., Bard, J., Chopra, R., Krishnamurthy, G., Mansour, T., O'Connell, J. (2008). Molecular Mechanism of Hepatitis C Virus Replicon Variants with Reduced Susceptibility to a Benzofuran Inhibitor, HCV-796. Antimicrob. Agents Chemother. 52: 3327-3338 [Abstract] [Full Text]  
  • Howe, A. Y. M., Cheng, H., Thompson, I., Chunduru, S. K., Herrmann, S., O'Connell, J., Agarwal, A., Chopra, R., Del Vecchio, A. M. (2006). Molecular Mechanism of a Thumb Domain Hepatitis C Virus Nonnucleoside RNA-Dependent RNA Polymerase Inhibitor. Antimicrob. Agents Chemother. 50: 4103-4113 [Abstract] [Full Text]  
  • Jouvencel, A.-C., Neau, D., Faure, M., Neau, M., Martinaud, C., Legrand, E., Trimoulet, P., Garrigue, I., Le Bail, B., Bioulac-Sage, P., Dupon, M., Ragnaud, J.-M., Fleury, H., Lafon, M.-E. (2006). Plasma and liver hepatitis C virus variability in patients coinfected with human immunodeficiency virus.. J. Clin. Microbiol. 44: 1877-1880 [Abstract] [Full Text]  
  • Jridi, C., Martin, J.-F., Marie-Jeanne, V., Labonne, G., Blanc, S. (2006). Distinct Viral Populations Differentiate and Evolve Independently in a Single Perennial Host Plant. J. Virol. 80: 2349-2357 [Abstract] [Full Text]  
  • Roque-Afonso, A.-M., Ducoulombier, D., Di Liberto, G., Kara, R., Gigou, M., Dussaix, E., Samuel, D., Feray, C. (2005). Compartmentalization of Hepatitis C Virus Genotypes between Plasma and Peripheral Blood Mononuclear Cells. J. Virol. 79: 6349-6357 [Abstract] [Full Text]  
  • Piron, M., Beguiristain, N., Nadal, A., Martinez-Salas, E., Gomez, J. (2005). Characterizing the function and structural organization of the 5' tRNA-like motif within the hepatitis C virus quasispecies. Nucleic Acids Res 33: 1487-1502 [Abstract] [Full Text]  
  • Cormier, E. G., Durso, R. J., Tsamis, F., Boussemart, L., Manix, C., Olson, W. C., Gardner, J. P., Dragic, T. (2004). L-SIGN (CD209L) and DC-SIGN (CD209) mediate transinfection of liver cells by hepatitis C virus. Proc. Natl. Acad. Sci. USA 101: 14067-14072 [Abstract] [Full Text]  
  • Forton, D. M., Karayiannis, P., Mahmud, N., Taylor-Robinson, S. D., Thomas, H. C. (2004). Identification of Unique Hepatitis C Virus Quasispecies in the Central Nervous System and Comparative Analysis of Internal Translational Efficiency of Brain, Liver, and Serum Variants. J. Virol. 78: 5170-5183 [Abstract] [Full Text]  
  • Cormier, E. G., Tsamis, F., Kajumo, F., Durso, R. J., Gardner, J. P., Dragic, T. (2004). CD81 is an entry coreceptor for hepatitis C virus. Proc. Natl. Acad. Sci. USA 101: 7270-7274 [Abstract] [Full Text]  
  • Wodarz, D. (2003). Hepatitis C virus dynamics and pathology: the role of CTL and antibody responses. J. Gen. Virol. 84: 1743-1750 [Abstract] [Full Text]  
  • Nadal, A., Robertson, H. D., Guardia, J., Gomez, J. (2003). Characterization of the structure and variability of an internal region of hepatitis C virus RNA for M1 RNA guide sequence ribozyme targeting. J. Gen. Virol. 84: 1545-1548 [Abstract] [Full Text]  
  • Nadal, A., Martell, M., Lytle, J. R., Lyons, A. J., Robertson, H. D., Cabot, B., Esteban, J. I., Esteban, R., Guardia, J., Gomez, J. (2002). Specific Cleavage of Hepatitis C Virus RNA Genome by Human RNase P. J. Biol. Chem. 277: 30606-30613 [Abstract] [Full Text]  
  • Curran, R., Jameson, C. L., Craggs, J. K., Grabowska, A. M., Thomson, B. J., Robins, A., Irving, W. L., Ball, J. K. (2002). Evolutionary trends of the first hypervariable region of the hepatitis C virus E2 protein in individuals with differing liver disease severity. J. Gen. Virol. 83: 11-23 [Abstract] [Full Text]  
  • Cabot, B., Martell, M., Esteban, J. I., Piron, M., Otero, T., Esteban, R., Guardia, J., Gomez, J. (2001). Longitudinal Evaluation of the Structure of Replicating and Circulating Hepatitis C Virus Quasispecies in Nonprogressive Chronic Hepatitis C Patients. J. Virol. 75: 12005-12013 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»