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Journal of Virology, October 2000, p. 9240-9244, Vol. 74, No. 19
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Phosphatidylserine-Mediated Phagocytosis of Influenza A Virus-Infected Cells by Mouse Peritoneal Macrophages

Akiko Shiratsuchi,1 Masako Kaido,1 Takenori Takizawa,2 and Yoshinobu Nakanishi1,*

Graduate School of Natural Science and Technology, Kanazawa University, Kanazawa, Ishikawa 920-0934,1 and Institute for Developmental Research, Aichi Human Service Center, Kasugai, Aichi 480-0392,2 Japan

Received 18 April 2000/Accepted 5 July 2000

Influenza virus induces apoptosis in cultured cell lines as well as in animal tissues. HeLa cells were infected with influenza virus A/Udon/72 (H3N2) under conditions resulting in almost 100% infection. Such cells underwent typical caspase-dependent apoptosis and were efficiently phagocytosed by macrophages prepared from peritoneal fluids of thioglycolate-treated mice. The membrane phospholipid phosphatidylserine appeared on the surfaces of virus-infected cells at around the time efficient phagocytosis became detectable. In fact, the phagocytosis was almost completely inhibited in the presence of liposomes containing phosphatidylserine, which did not influence the antibody-dependent uptake of zymosan particles by the same macrophages. These results indicate that macrophages phagocytose influenza virus-infected HeLa cells in a manner mediated by phosphatidylserine that appears on the surfaces of infected cells during the process of apoptosis.

* Corresponding author. Mailing address: Graduate School of Natural Science and Technology, Kanazawa University, Takara-machi, Kanazawa, Ishikawa 920-0934, Japan. Phone: 81-76-234-4481. Fax: 81-76-234-4480. E-mail: nakanaka{at}kenroku.kanazawa-u.ac.jp.

Journal of Virology, October 2000, p. 9240-9244, Vol. 74, No. 19
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Hashimoto, Y., Moki, T., Takizawa, T., Shiratsuchi, A., Nakanishi, Y. (2007). Evidence for Phagocytosis of Influenza Virus-Infected, Apoptotic Cells by Neutrophils and Macrophages in Mice. J. Immunol. 178: 2448-2457 [Abstract] [Full Text]  
  • Watanabe, Y., Shiratsuchi, A., Shimizu, K., Takizawa, T., Nakanishi, Y. (2002). Role of Phosphatidylserine Exposure and Sugar Chain Desialylation at the Surface of Influenza Virus-infected Cells in Efficient Phagocytosis by Macrophages. J. Biol. Chem. 277: 18222-18228 [Abstract] [Full Text]  
  • Uchide, N., Ohyama, K., Yuan, B., Bessho, T., Yamakawa, T. (2002). Differentiation of monocytes to macrophages induced by influenza virus-infected apoptotic cells. J. Gen. Virol. 83: 747-751 [Abstract] [Full Text]  


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