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Journal of Virology, October 2000, p. 9083-9089, Vol. 74, No. 19
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Induction of CD8 T Cells by Vaccination with Recombinant Adenovirus Expressing Human Papillomavirus Type 16 E5 Gene Reduces Tumor Growth

Dai-Wei Liu,1,2 Yeou-Ping Tsao,1,3 Chang-Hsun Hsieh,1 Jer-Tsong Hsieh,4 John T. Kung,5 Chia-Lien Chiang,1 Shyh-Jer Huang,1 and Show-Li Chen1,*

Department of Microbiology and Immunology1 and The Graduate Institute of Medical Science,2 National Defense Medical Center, and Institute of Molecular Biology, Academia Sinica,5 Taipei, and Department of Ophthalmology, Chang Gung Memorial Hospital and Chang Gung University, Taoyuan,3 Taiwan, Republic of China, and Department of Urology, The Southwestern Medical School, Dallas, Texas4

Received 2 May 2000/Accepted 14 July 2000

The potential of the E5 protein as a tumor vaccine candidate has not been explored yet. In this study, we evaluate the human papillomavirus type 16 (HPV-16) E5 protein delivered by an adenovirus vector as a tumor vaccine for cervical lesions. The results demonstrate that a single intramuscular injection of a recombinant adenovirus carrying the HPV-16 E5 gene into syngeneic animals can reduce the growth of tumors which contain E5 gene expression. Moreover, the E5 vaccine-induced tumor protection occurs through CD8 T cells but not through CD4 T cells in in vitro assays. In addition, our studies using knockout mice with distinct T-cell deficiencies confirm that cytotoxic T-lymphocyte-induced tumor protection is CD8 dependent but CD4 independent. Hence, HPV-16 E5 can be regarded as a tumor rejection antigen.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, National Defense Medical Center, Taipei, Taiwan, Republic of China. Phone: 886-2-87923100, ext. 18543. Fax: 886-2-87924885. E-mail: yptsao{at}kimo.com.tw.

Journal of Virology, October 2000, p. 9083-9089, Vol. 74, No. 19
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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