This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Das, S. C.
Right arrow Articles by Barrett, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Das, S. C.
Right arrow Articles by Barrett, T.

 Previous Article  |  Next Article 

Journal of Virology, October 2000, p. 9039-9047, Vol. 74, No. 19
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Recovery and Characterization of a Chimeric Rinderpest Virus with the Glycoproteins of Peste-des-Petits-Ruminants Virus: Homologous F and H Proteins Are Required for Virus Viability

Subash C. Das,dagger Michael D. Baron, and Thomas Barrett*

Institute for Animal Health, Pirbright, Surrey GU24 0NF, United Kingdom

Received 9 February 2000/Accepted 10 July 2000

Rinderpest (RP) and peste-des-petits-ruminants (PPR) are two important diseases of domestic ruminants. To improve on currently available vaccines against PPR, we have created cDNA copies of the RP virus genome in which either the fusion (F) or hemagglutinin (H) gene, or both, was replaced with the corresponding gene from PPR virus. It was necessary to develop a modified rescue system in which the T7 RNA polymerase was provided by a recombinant fowlpox virus and the entire rescue procedure took place in Vero cells before we could obtain live virus from these chimeric constructs. No virus was recovered when only one of the glycoprotein genes was changed, but a chimeric virus containing both F and H genes from PPR virus was reproducibly rescued from cDNA, indicating that a virus-specific functional interaction takes place between the F and H proteins. The rescued virus expressing the PPR glycoproteins grew more slowly in tissue culture than either parental virus and formed abnormally large syncytia. Goats infected with the chimera showed no adverse reaction, as assessed by clinical signs, temperature, leukocyte count, virus isolation, and serology, and were protected from subsequent challenge with wild-type PPR virus.

* Corresponding author. Mailing address: Institute for Animal Health, Ash Road, Pirbright, Surrey GU24 0NF, United Kingdom. Phone: 44 1483 231024. Fax: 44 (0)1483 232448. E-mail: tom.barrett{at}bbsrc.ac.uk.

dagger Present address: Division of Animal Health, Central Sheep and Wool Research Institute, Avikanagar (Via: Jaipur), 304501 Rajasthan, India.

Journal of Virology, October 2000, p. 9039-9047, Vol. 74, No. 19
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Parida, S., Mahapatra, M., Kumar, S., Das, S. C., Baron, M. D., Anderson, J., Barrett, T. (2007). Rescue of a chimeric rinderpest virus with the nucleocapsid protein derived from peste-des-petits-ruminants virus: use as a marker vaccine. J. Gen. Virol. 88: 2019-2027 [Abstract] [Full Text]  
  • Nanda, S. K., Baron, M. D. (2006). Rinderpest Virus Blocks Type I and Type II Interferon Action: Role of Structural and Nonstructural Proteins.. J. Virol. 80: 7555-7568 [Abstract] [Full Text]  
  • Mahapatra, M., Parida, S., Baron, M. D., Barrett, T. (2006). Matrix protein and glycoproteins F and H of Peste-des-petits-ruminants virus function better as a homologous complex. J. Gen. Virol. 87: 2021-2029 [Abstract] [Full Text]  
  • Pham, Q. N., Biacchesi, S., Skiadopoulos, M. H., Murphy, B. R., Collins, P. L., Buchholz, U. J. (2005). Chimeric Recombinant Human Metapneumoviruses with the Nucleoprotein or Phosphoprotein Open Reading Frame Replaced by That of Avian Metapneumovirus Exhibit Improved Growth In Vitro and Attenuation In Vivo. J. Virol. 79: 15114-15122 [Abstract] [Full Text]  
  • Banyard, A. C., Baron, M. D., Barrett, T. (2005). A role for virus promoters in determining the pathogenesis of Rinderpest virus in cattle. J. Gen. Virol. 86: 1083-1092 [Abstract] [Full Text]  
  • Baron, M. D., Banyard, A. C., Parida, S., Barrett, T. (2005). The Plowright vaccine strain of Rinderpest virus has attenuating mutations in most genes. J. Gen. Virol. 86: 1093-1101 [Abstract] [Full Text]  
  • Neumann, G., Whitt, M. A., Kawaoka, Y. (2002). A decade after the generation of a negative-sense RNA virus from cloned cDNA - what have we learned?. J. Gen. Virol. 83: 2635-2662 [Abstract] [Full Text]  
  • Yoneda, M., Bandyopadhyay, S. K., Shiotani, M., Fujita, K., Nuntaprasert, A., Miura, R., Baron, M. D., Barrett, T., Kai, C. (2002). Rinderpest virus H protein: role in determining host range in rabbits. J. Gen. Virol. 83: 1457-1463 [Abstract] [Full Text]  
  • Takeuchi, K., Takeda, M., Miyajima, N., Kobune, F., Tanabayashi, K., Tashiro, M. (2002). Recombinant Wild-Type and Edmonston Strain Measles Viruses Bearing Heterologous H Proteins: Role of H Protein in Cell Fusion and Host Cell Specificity. J. Virol. 76: 4891-4900 [Abstract] [Full Text]  
  • Stope, M. B., Karger, A., Schmidt, U., Buchholz, U. J. (2001). Chimeric Bovine Respiratory Syncytial Virus with Attachment and Fusion Glycoproteins Replaced by Bovine Parainfluenza Virus Type 3 Hemagglutinin-Neuraminidase and Fusion Proteins. J. Virol. 75: 9367-9377 [Abstract] [Full Text]  
  • von Messling, V., Zimmer, G., Herrler, G., Haas, L., Cattaneo, R. (2001). The Hemagglutinin of Canine Distemper Virus Determines Tropism and Cytopathogenicity. J. Virol. 75: 6418-6427 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»