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Journal of Virology, August 2000, p. 7687-7690, Vol. 74, No. 16
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Infection of Nonhuman Primate Cells by Pig Endogenous Retrovirus

Juergen H. Blusch,1 Clive Patience,2,dagger Yasuhiro Takeuchi,2,Dagger Christian Templin,3 Christian Roos,4 Klaus Von Der Helm,1 Gustav Steinhoff,3 and Ulrich Martin3,*

Max von Pettenkofer Institute, Department of Virology,1 and Department of Medical Genetics,4 Ludwig Maximilian University, Munich, and Leibniz Research Laboratories for Biotechnology and Artificial Organs, Hannover Medical School, 30659 Hannover,3 Germany, and Chester Beatty Laboratories, Institute of Cancer Research, London SW3 6JB, United Kingdom2

Received 14 March 2000/Accepted 22 May 2000

The ongoing shortage of human donor organs for transplantation has catalyzed new interest in the application of pig organs (xenotransplantation). One of the biggest concerns about the transplantation of porcine grafts into humans is the transmission of pig endogenous retroviruses (PERV) to the recipients or even to other members of the community. Although nonhuman primate models are excellently suited to mimic clinical xenotransplantation settings, their value for risk assessment of PERV transmission at xenotransplantation is questionable since all of the primate cell lines tested so far have been found to be nonpermissive for PERV infection. Here we demonstrate that human, gorilla, and Papio hamadryas primary skin fibroblasts and also baboon B-cell lines are permissive for PERV infection. This suggests that a reevaluation of the suitability of the baboon model for risk assessment in xenotransplantation is critical at this point.

* Corresponding author. Mailing address: Leibniz Research Laboratories for Biotechnology and Artificial Organs, Hannover Medical School, Podbielskistr. 380, D-30659 Hannover, Germany. Phone: 49-511-906-3533. Fax: 49-511-906-3569. E-mail: umartin{at}artificial-organs.de.

dagger Present address: BioTransplant Incorporated, Charlestown, MA 02129.

Dagger Present address: Wohl Virion Centre, Windeyer Institute, University College London, London W1P 6DB, United Kingdom.

Journal of Virology, August 2000, p. 7687-7690, Vol. 74, No. 16
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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