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Journal of Virology, August 2000, p. 7642-7645, Vol. 74, No. 16
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Apical Gene Transfer into Quiescent Human and Canine Polarized Intestinal Epithelial Cells by Lentivirus Vectors

Jurgen Seppen,1,dagger Simon C. Barry,1 J. Henriette Klinkspoor,2 Louis J. Katen,1 Sum P. Lee,2,3 J. Victor Garcia,4 and William R. A. Osborne1,*

Department of Pediatrics, School of Medicine,1 and Department of Gastroenterology,2 University of Washington, and Veterans Affairs Medical Center,3 Seattle, Washington, and Department of Internal Medicine, Southwestern Medical Center, Dallas, Texas4

Received 10 August 1999/Accepted 4 May 2000

Intestinal epithelial cells secrete a protective luminal mucus barrier inhibiting viral gene transfer. Quiescent, polarized monolayers of primary epithelial cells from dog gallbladder and human colon are efficiently transduced through the apical mucus side by lentivirus vectors, suggesting their application to intestinal gene therapy.

* Corresponding author. Mailing address: Department of Pediatrics, University of Washington School of Medicine, Box 356320, Seattle, WA 98195-6320. Phone: (206) 543-4735. Fax: (206) 543-3184. E-mail: wosborne{at}u.washington.edu.

dagger Present address: Academic Medical Center, Department of Experimental Hepatology, 1105 AZ Amsterdam, Netherlands.

Journal of Virology, August 2000, p. 7642-7645, Vol. 74, No. 16
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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