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Journal of Virology, August 2000, p. 7338-7348, Vol. 74, No. 16
0022-538X/00/$04.00+0

Viral Activation of Interleukin-15 (IL-15): Characterization of a Virus-Inducible Element in the IL-15 Promoter Region

Nazli Azimi,* Yutaka Tagaya, Jennifer Mariner, and Thomas A. Waldmann

Metabolism Branch, Division of Clinical Sciences, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892-1374

Received 7 February 2000/Accepted 18 May 2000

We identified an interferon regulatory factor motif (IRF-E) upstream of an NF-kappa B binding site in the interleukin-15 (IL-15) promoter. Since these two motifs are part of the virus-inducible enhancer region of the beta interferon promoter, we speculated that there might be similar responses of these two genes to stimuli such as viruses. To test this hypothesis, L929 cells were infected with Newcastle disease virus (NDV), which led to the induction of IL-15 mRNA and protein expression. Using IL-15 promoter-reporter deletion constructs, a virus-inducible region, encompassing IRF-E, NF-kappa B, and a 13-nucleotide sequence flanked by these two motifs, was mapped to the -295-to--243 position relative to the transcription initiation site. Using cotransfection studies, it was demonstrated that all three motifs were essential to achieve the maximum promoter activity induced by IRF-1 and NF-kappa B expression plasmids. The presence of a virus-inducible region in the IL-15 promoter suggests a role for IL-15 as a component of host antiviral defense mechanisms.

* Corresponding author. Mailing address: Metabolism Branch, Division of Clinical Sciences, Bldg. 10, Rm. 4N-102, National Cancer Institute, National Institutes of Health, 10 Center Drive MSC 1374, Bethesda, MD 20892-1374. Phone: (301) 496-8382. Fax: (301) 496-9956. E-mail: nazli{at}helix.nih.gov.

Journal of Virology, August 2000, p. 7338-7348, Vol. 74, No. 16
0022-538X/00/$04.00+0


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