This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Zemskov, E. A.
Right arrow Articles by Maeda, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zemskov, E. A.
Right arrow Articles by Maeda, S.

 Previous Article  |  Next Article 

Journal of Virology, August 2000, p. 6784-6789, Vol. 74, No. 15
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Evidence for Nucleic Acid Binding Ability and Nucleosome Association of Bombyx mori Nucleopolyhedrovirus BRO Proteins

Evgueni A. Zemskov,1,2 WonKyung Kang,1,* and Susumu Maeda1,3,dagger

Laboratory of Molecular Entomology and Baculovirology1 and Laboratory of CAG Repeat Diseases, Brain Science Institute,2 RIKEN, 2-1 Hirosawa, Wako 351-0198, and Core Research for Evolutional Science and Technology Project, Japan Science and Technology Corporation, Kawaguchi,3 Japan

Received 22 February 2000/Accepted 2 May 2000

The Bombyx mori nucleopolyhedrovirus (BmNPV) genome contains five related members of the bro gene family, all of which are actively expressed in infected BmN cells. Although their functions are unknown, their amino acid sequences contain a motif found in all known viral and prokaryotic single-stranded DNA binding proteins. To determine if they bind to nucleic acids, we fractionated the nuclei of BmNPV-infected BmN cells using a histone extraction protocol. We detected BRO-A, BRO-C, and BRO-D in the histone H1 fraction using anti-BRO antibodies. Micrococcal nuclease treatment released these BRO proteins from the chromatin fraction, suggesting their involvement in nucleosome structures. Chromatographic fractionation showed that BRO-A and/or BRO-C interacted with core histones. Expression of partial sequences of BRO-A proved that the N-terminal 80 amino acid residues were required for DNA binding activity. We also demonstrated that BmNPV BRO proteins underwent phosphorylation and ubiquitination followed by proteasome degradation, which may explain their distribution in the cytoplasm as well as the nucleus. We propose that BRO-A and BRO-C may function as DNA binding proteins that influence host DNA replication and/or transcription.

* Corresponding author. Mailing address: Laboratory of Molecular Entomology and Baculovirology, RIKEN, 2-1 Hirosawa, Wako 351-0198, Japan. Phone: 81-48-467-9584. Fax: 81-48-462-4678. E-mail: wkkang{at}postman.riken.go.jp.

dagger Deceased.

Journal of Virology, August 2000, p. 6784-6789, Vol. 74, No. 15
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Eberle, K. E., Sayed, S., Rezapanah, M., Shojai-Estabragh, S., Jehle, J. A. (2009). Diversity and evolution of the Cydia pomonella granulovirus. J. Gen. Virol. 90: 662-671 [Abstract] [Full Text]  
  • Hilton, S., Winstanley, D. (2008). Genomic sequence and biological characterization of a nucleopolyhedrovirus isolated from the summer fruit tortrix, Adoxophyes orana. J. Gen. Virol. 89: 2898-2908 [Abstract] [Full Text]  
  • Harrison, R. L., Puttler, B., Popham, H. J. R. (2008). Genomic sequence analysis of a fast-killing isolate of Spodoptera frugiperda multiple nucleopolyhedrovirus. J. Gen. Virol. 89: 775-790 [Abstract] [Full Text]  
  • Asgari, S., Davis, J., Wood, D., Wilson, P., McGrath, A. (2007). Sequence and organization of the Heliothis virescens ascovirus genome. J. Gen. Virol. 88: 1120-1132 [Abstract] [Full Text]  
  • Bideshi, D. K., Demattei, M.-V., Rouleux-Bonnin, F., Stasiak, K., Tan, Y., Bigot, S., Bigot, Y., Federici, B. A. (2006). Genomic Sequence of Spodoptera frugiperda Ascovirus 1a, an Enveloped, Double-Stranded DNA Insect Virus That Manipulates Apoptosis for Viral Reproduction. J. Virol. 80: 11791-11805 [Abstract] [Full Text]  
  • Oliveira, J. V. d. C., Wolff, J. L. C., Garcia-Maruniak, A., Ribeiro, B. M., de Castro, M. E. B., de Souza, M. L., Moscardi, F., Maruniak, J. E., Zanotto, P. M. d. A. (2006). Genome of the most widely used viral biopesticide: Anticarsia gemmatalis multiple nucleopolyhedrovirus.. J. Gen. Virol. 87: 3233-3250 [Abstract] [Full Text]  
  • Delhon, G., Tulman, E. R., Afonso, C. L., Lu, Z., Becnel, J. J., Moser, B. A., Kutish, G. F., Rock, D. L. (2006). Genome of invertebrate iridescent virus type 3 (mosquito iridescent virus).. J. Virol. 80: 8439-8449 [Abstract] [Full Text]  
  • Duffy, S. P., Young, A. M., Morin, B., Lucarotti, C. J., Koop, B. F., Levin, D. B. (2006). Sequence Analysis and Organization of the Neodiprion abietis Nucleopolyhedrovirus Genome. J. Virol. 80: 6952-6963 [Abstract] [Full Text]  
  • Lauzon, H. A. M., Jamieson, P. B., Krell, P. J., Arif, B. M. (2005). Gene organization and sequencing of the Choristoneura fumiferana defective nucleopolyhedrovirus genome. J. Gen. Virol. 86: 945-961 [Abstract] [Full Text]  
  • Imai, N., Matsumoto, S., Kang, W. (2005). Formation of Bombyx mori nucleopolyhedrovirus IE2 nuclear foci is regulated by the functional domains for oligomerization and ubiquitin ligase activity. J. Gen. Virol. 86: 637-644 [Abstract] [Full Text]  
  • Li, L., Li, Q., Willis, L. G., Erlandson, M., Theilmann, D. A., Donly, C. (2005). Complete comparative genomic analysis of two field isolates of Mamestra configurata nucleopolyhedrovirus-A. J. Gen. Virol. 86: 91-105 [Abstract] [Full Text]  
  • Bideshi, D. K., Renault, S., Stasiak, K., Federici, B. A., Bigot, Y. (2003). Phylogenetic analysis and possible function of bro-like genes, a multigene family widespread among large double-stranded DNA viruses of invertebrates and bacteria. J. Gen. Virol. 84: 2531-2544 [Abstract] [Full Text]  
  • Harrison, R. L., Bonning, B. C. (2003). Comparative analysis of the genomes of Rachiplusia ou and Autographa californica multiple nucleopolyhedroviruses. J. Gen. Virol. 84: 1827-1842 [Abstract] [Full Text]  
  • Iyer, L. M., Aravind, L., Koonin, E. V. (2001). Common Origin of Four Diverse Families of Large Eukaryotic DNA Viruses. J. Virol. 75: 11720-11734 [Abstract] [Full Text]  
  • Afonso, C. L., Tulman, E. R., Lu, Z., Balinsky, C. A., Moser, B. A., Becnel, J. J., Rock, D. L., Kutish, G. F. (2001). Genome Sequence of a Baculovirus Pathogenic for Culex nigripalpus. J. Virol. 75: 11157-11165 [Abstract] [Full Text]  
  • Acharya, A., Gopinathan, K. P. (2001). Identification of an enhancer-like element in the polyhedrin gene upstream region of Bombyx mori nucleopolyhedrovirus. J. Gen. Virol. 82: 2811-2819 [Abstract] [Full Text]  
  • Luque, T., Finch, R., Crook, N., O'Reilly, D. R., Winstanley, D. (2001). The complete sequence of the Cydia pomonella granulovirus genome. J. Gen. Virol. 82: 2531-2547 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»