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Journal of Virology, July 2000, p. 6556-6563, Vol. 74, No. 14
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Cowpea Mosaic Virus Infection Induces a Massive Proliferation of Endoplasmic Reticulum but Not Golgi Membranes and Is Dependent on De Novo Membrane Synthesis

Jan E. Carette,1 Marchel Stuiver,1 Jan Van Lent,2 Joan Wellink,1,* and Ab Van Kammen1

Laboratory of Molecular Biology, Wageningen University, 6703 HA Wageningen,1 and Laboratory of Virology, Wageningen University, 6709 PD Wageningen,2 The Netherlands

Received 28 December 1999/Accepted 17 April 2000

Replication of cowpea mosaic virus (CPMV) is associated with small membranous vesicles that are induced upon infection. The effect of CPMV replication on the morphology and distribution of the endomembrane system in living plant cells was studied by expressing green fluorescent protein (GFP) targeted to the endoplasmic reticulum (ER) and the Golgi membranes. CPMV infection was found to induce an extensive proliferation of the ER, whereas the distribution and morphology of the Golgi stacks remained unaffected. Immunolocalization experiments using fluorescence confocal microscopy showed that the proliferated ER membranes were closely associated with the electron-dense structures that contain the replicative proteins encoded by RNA1. Replication of CPMV was strongly inhibited by cerulenin, an inhibitor of de novo lipid synthesis, at concentrations where the replication of the two unrelated viruses alfalfa mosaic virus and tobacco mosaic virus was largely unaffected. These results suggest that proliferating ER membranes produce the membranous vesicles formed during CPMV infection and that this process requires continuous lipid biosynthesis.

* Corresponding author. Mailing address: Wageningen University, Laboratory of Molecular Biology, Dreijenlaan 3, 6703 HA Wageningen, The Netherlands. Phone: 31-317483266. Fax: 31-317483584. E-mail: joan.wellink{at}mac.mb.wau.nl.

Journal of Virology, July 2000, p. 6556-6563, Vol. 74, No. 14
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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