This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Owen, S. M.
Right arrow Articles by Lal, R. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Owen, S. M.
Right arrow Articles by Lal, R. B.

 Previous Article  |  Next Article 

Journal of Virology, June 2000, p. 5702-5708, Vol. 74, No. 12
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Simian Immunodeficiency Viruses of Diverse Origin Can Use CXCR4 as a Coreceptor for Entry into Human Cells

Sherry M. Owen,1 Silvina Masciotra,1 Frank Novembre,2 JoAnn Yee,3 William M. Switzer,1 Moses Ostyula,4 and Renu B. Lal1,*

HIV and Retrovirology Branch, Division of AIDS, STD, and TB Laboratory Research, National Center for Infectious Diseases, Centers for Disease Control and Prevention,1 and Yerkes Primate Center, Emory University,2 Atlanta, Georgia; California Regional Primate Research Center, University of California, Davis, California3; and Virology Division, Institute of Primate Research, Nairobi, Kenya4

Received 24 November 1999/Accepted 29 March 2000

Primary simian immunodeficiency virus (SIV) isolated from sooty mangabey (SIVsm [n = 6]), stumptail (SIVstm [n = 1]), mandrill (SIVmnd [n = 1]), and African green (SIVagm [n = 1]) primates were examined for their ability to infect human cells and for their coreceptor requirements. All isolates infected human peripheral blood mononuclear cells (PBMCs) from a CCR5+/+ donor, and seven of eight isolates tested also infected CCR5-/- PBMCs. Analysis of coreceptor utilization using GHOST and U87 cell lines revealed that all of the isolates tested used CCR5 and the orphan receptors STRL33 and GPR15. Coreceptors such as CCR2b, CCR3, CCR8, and CX3CR1 were also utilized by some primary SIV isolates. More importantly, we found that CXCR4 was used as a coreceptor by the SIVstm, the SIVagm, and four of the SIVsm isolates in GHOST and U87 cells. These data suggest that primary SIV isolates from diverse primate species can utilize CXCR4 for viral entry, similar to what has been described for human immunodeficiency viruses.

* Corresponding author. Mailing address: MS D-12, HIV Immunology and Diagnostics Branch, CDC, 1600 Clifton Rd., Atlanta, GA 30333. Phone: (404) 639-1036. Fax: (404) 639-2660. E-mail: rbl3{at}cdc.gov.

Journal of Virology, June 2000, p. 5702-5708, Vol. 74, No. 12
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Del Prete, G. Q., Haggarty, B., Leslie, G. J., Jordan, A. P. O., Romano, J., Wang, N., Wang, J., Holmes, M. C., Montefiori, D. C., Hoxie, J. A. (2009). Derivation and Characterization of a Simian Immunodeficiency Virus SIVmac239 Variant with Tropism for CXCR4. J. Virol. 83: 9911-9922 [Abstract] [Full Text]  
  • MacFie, T.S., Nerrienet, E., de Groot, N.G., Bontrop, R.E., Mundy, N.I. (2009). Patterns of Diversity in HIV-Related Loci among Subspecies of Chimpanzee: Concordance at CCR5 and Differences at CXCR4 and CX3CR1. Mol Biol Evol 26: 719-727 [Abstract] [Full Text]  
  • Cameron, P. U., Handley, A. J., Baylis, D. C., Solomon, A. E., Bernard, N., Purcell, D. F. J., Lewin, S. R. (2007). Preferential Infection of Dendritic Cells during Human Immunodeficiency Virus Type 1 Infection of Blood Leukocytes. J. Virol. 81: 2297-2306 [Abstract] [Full Text]  
  • VandeWoude, S., Apetrei, C. (2006). Going Wild: Lessons from Naturally Occurring T-Lymphotropic Lentiviruses. Clin. Microbiol. Rev. 19: 728-762 [Abstract] [Full Text]  
  • Lauren, A., Vodros, D., Thorstensson, R., Fenyo, E. M. (2006). Comparative studies on mucosal and intravenous transmission of simian immunodeficiency virus (SIVsm): evolution of coreceptor use varies with pathogenic outcome.. J. Gen. Virol. 87: 581-594 [Abstract] [Full Text]  
  • Takeuchi, H., Kao, S., Miyagi, E., Khan, M. A., Buckler-White, A., Plishka, R., Strebel, K. (2005). Production of Infectious SIVagm from Human Cells Requires Functional Inactivation but Not Viral Exclusion of Human APOBEC3G. J. Biol. Chem. 280: 375-382 [Abstract] [Full Text]  
  • Bogerd, H. P., Doehle, B. P., Wiegand, H. L., Cullen, B. R. (2004). From The Cover: A single amino acid difference in the host APOBEC3G protein controls the primate species specificity of HIV type 1 virion infectivity factor. Proc. Natl. Acad. Sci. USA 101: 3770-3774 [Abstract] [Full Text]  
  • Ling, B., Santiago, M. L., Meleth, S., Gormus, B., McClure, H. M., Apetrei, C., Hahn, B. H., Marx, P. A. (2003). Noninvasive Detection of New Simian Immunodeficiency Virus Lineages in Captive Sooty Mangabeys: Ability To Amplify Virion RNA from Fecal Samples Correlates with Viral Load in Plasma. J. Virol. 77: 2214-2226 [Abstract] [Full Text]  
  • Konig, R. R., Flory, E., Steidl, S., Neumann, J., Coulibaly, C., Holznagel, E., Holzammer, S., Norley, S., Cichutek, K. (2002). Engineered CD4- and CXCR4-Using Simian Immunodeficiency Virus from African Green Monkeys Is Neutralization Sensitive and Replicates in Nonstimulated Lymphocytes. J. Virol. 76: 10627-10636 [Abstract] [Full Text]  
  • Masciotra, S., Yang, C., Pieniazek, D., Thomas, C., Owen, S. M., McClure, H. M., Lal, R. B. (2002). Detection of Simian Immunodeficiency Virus in Diverse Species and of Human Immunodeficiency Virus Type 2 by Using Consensus Primers within the pol Region. J. Clin. Microbiol. 40: 3167-3171 [Abstract] [Full Text]  
  • Clapham, P. R., McKnight, A. (2002). Cell surface receptors, virus entry and tropism of primate lentiviruses. J. Gen. Virol. 83: 1809-1829 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»