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Journal of Virology, June 2000, p. 5655-5658, Vol. 74, No. 12
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Isolation and Characterization of a New Subtype of Borna Disease Virus

Norbert Nowotny,1,* Jolanta Kolodziejek,1 Christian O. Jehle,2 Angelika Suchy,3 Peter Staeheli,2 and Martin Schwemmle2

Institute of Virology1 and Institute of Pathology and Forensic Veterinary Medicine,3 University of Veterinary Sciences, Vienna, A-1210 Vienna, Austria, and Department of Virology, University of Freiburg, D-79008 Freiburg, Germany2

Received 13 December 1999/Accepted 18 March 2000

Borna disease virus (BDV), the causative agent of severe meningoencephalitis in a wide variety of animal species, has been considered to be genetically invariable and to form a single type within the genus Bornavirus of the family Bornaviridae. BDV infections are of particular interest, because for the first time a virus infection appears to be linked to human psychiatric disorders. We now describe a new subtype of BDV isolated from a horse which was euthanatized due to severe, incurable neurological disease. The nucleotide sequence of this new strain, named No/98, differs from the reference strains by more than 15%, and the subtype is difficult to detect by standard reverse transcriptase PCR protocols. The nucleotide exchanges of the novel BDV isolate have surprisingly little effect on the primary structures of most viral proteins, with the notable exception of the X protein (p10), which is only 81% identical to its counterpart in reference strains. Our data indicate that the genome of BDV is far more variable than previously assumed and that naturally occurring subtypes may escape detection by currently used diagnostic assays.

* Corresponding author. Mailing address: Institute of Virology, University of Veterinary Sciences, Vienna, Veterinärplatz 1, A-1210 Vienna, Austria. Phone: 43 1 25077-2304. Fax: 43 1 25077-2390. E-mail: Norbert.Nowotny{at}vu-wien.ac.at.

Journal of Virology, June 2000, p. 5655-5658, Vol. 74, No. 12
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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