This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Okamoto, H.
Right arrow Articles by Mayumi, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Okamoto, H.
Right arrow Articles by Mayumi, M.

 Previous Article  |  Next Article 

Journal of Virology, June 2000, p. 5161-5167, Vol. 74, No. 11
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Circular Double-Stranded Forms of TT Virus DNA in the Liver

Hiroaki Okamoto,1 Masato Ukita,1 Tsutomu Nishizawa,1 Junichi Kishimoto,2 Yuji Hoshi,3 Hitoshi Mizuo,4 Takeshi Tanaka,3 Yuzo Miyakawa,5 and Makoto Mayumi1,*

Immunology Division and Division of Molecular Virology, Jichi Medical School, Tochigi-Ken 329-0498,1 Institute of Immunology, Tokyo 112-0004,2 Japanese Red Cross Saitama Blood Center, Saitama-Ken 338-0001,3 Department of Internal Medicine, Kin-ikyo Chuo Hospital, Hokkaido 007-0870,4 and Miyakawa Memorial Research Foundation, Tokyo 107-0062,5 Japan

Received 8 December 1999/Accepted 13 March 2000

TT virus (TTV) is an unenveloped, circular, and single-stranded DNA virus commonly infecting human beings worldwide. TTV DNAs in paired serum and liver tissues from three viremic individuals were separated by gel electrophoresis and characterized biophysically. TTV DNAs in sera migrated in sizes ranging from 2.0 to 2.5 kb. TTV DNAs in liver tissues, however, migrated at 2.0 to 2.5 kb as well as at 3.5 to 6.1 kb. Both faster- and slower-migrating forms of TTV DNAs in the liver were found to be circular and of the full genomic length of 3.8 kb. TTV DNAs migrating at 2.0 to 2.5 kb, from either serum or liver tissues, were sensitive to S1 nuclease but resistant to restriction endonucleases, and therefore, they were single-stranded. By contrast, TTV DNAs in liver tissues that migrated at 3.5 to 6.1 kb were resistant to S1 nuclease. They migrated at 3.7 to 4.0 kb after digestion with EcoRI, which suggests that they represent circular, double-stranded replicative intermediates of TTV. When TTV DNAs were subjected to strand-specific primer extension and then amplified by PCR with internal primers, those in serum were found to be minus-stranded DNAs while those in liver tissues were found to be a mixture of plus- and minus-stranded DNAs. These results suggest that TTV replicates in the liver via a circular double-stranded DNA.

* Corresponding author. Mailing address: Minamikawachi-Machi, Tochigi-Ken 329-0498, Japan. Phone: 81-285-58-7404. Fax: 81-285-44-1557. E-mail: immundiv{at}jichi.ac.jp.

Journal of Virology, June 2000, p. 5161-5167, Vol. 74, No. 11
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Ng, T. F. F., Suedmeyer, W. K., Wheeler, E., Gulland, F., Breitbart, M. (2009). Novel anellovirus discovered from a mortality event of captive California sea lions. J. Gen. Virol. 90: 1256-1261 [Abstract] [Full Text]  
  • Ng, T. F. F., Manire, C., Borrowman, K., Langer, T., Ehrhart, L., Breitbart, M. (2009). Discovery of a Novel Single-Stranded DNA Virus from a Sea Turtle Fibropapilloma by Using Viral Metagenomics. J. Virol. 83: 2500-2509 [Abstract] [Full Text]  
  • Leppik, L., Gunst, K., Lehtinen, M., Dillner, J., Streker, K., de Villiers, E.-M. (2007). In Vivo and In Vitro Intragenomic Rearrangement of TT Viruses. J. Virol. 81: 9346-9356 [Abstract] [Full Text]  
  • Jelcic, I., Hotz-Wagenblatt, A., Hunziker, A., zur Hausen, H., de Villiers, E.-M. (2004). Isolation of Multiple TT Virus Genotypes from Spleen Biopsy Tissue from a Hodgkin's Disease Patient: Genome Reorganization and Diversity in the Hypervariable Region. J. Virol. 78: 7498-7507 [Abstract] [Full Text]  
  • Okamoto, H., Takahashi, M., Nishizawa, T., Tawara, A., Fukai, K., Muramatsu, U., Naito, Y., Yoshikawa, A. (2002). Genomic characterization of TT viruses (TTVs) in pigs, cats and dogs and their relatedness with species-specific TTVs in primates and tupaias. J. Gen. Virol. 83: 1291-1297 [Abstract] [Full Text]  
  • Yokoyama, H., Yasuda, J., Okamoto, H., Iwakura, Y. (2002). Pathological changes of renal epithelial cells in mice transgenic for the TT virus ORF1 gene. J. Gen. Virol. 83: 141-150 [Abstract] [Full Text]  
  • Maggi, F., Pistello, M., Vatteroni, M., Presciuttini, S., Marchi, S., Isola, P., Fornai, C., Fagnani, S., Andreoli, E., Antonelli, G., Bendinelli, M. (2001). Dynamics of Persistent TT Virus Infection, as Determined in Patients Treated with Alpha Interferon for Concomitant Hepatitis C Virus Infection. J. Virol. 75: 11999-12004 [Abstract] [Full Text]  
  • Okamoto, H., Nishizawa, T., Takahashi, M., Tawara, A., Peng, Y., Kishimoto, J., Wang, Y. (2001). Genomic and evolutionary characterization of TT virus (TTV) in tupaias and comparison with species-specific TTVs in humans and non-human primates. J. Gen. Virol. 82: 2041-2050 [Abstract] [Full Text]  
  • Bendinelli, M., Pistello, M., Maggi, F., Fornai, C., Freer, G., Vatteroni, M. L. (2001). Molecular Properties, Biology, and Clinical Implications of TT Virus, a Recently Identified Widespread Infectious Agent of Humans. Clin. Microbiol. Rev. 14: 98-113 [Abstract] [Full Text]  
  • Okamoto, H., Takahashi, M., Kato, N., Fukuda, M., Tawara, A., Fukuda, S., Tanaka, T., Miyakawa, Y., Mayumi, M. (2000). Sequestration of TT Virus of Restricted Genotypes in Peripheral Blood Mononuclear Cells. J. Virol. 74: 10236-10239 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»