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Journal of Virology, June 2000, p. 4999-5005, Vol. 74, No. 11
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Induction of CD4+ T-Cell-Independent Immunoglobulin Responses by Inactivated Influenza Virus

Zhiyi Sha and Richard W. Compans*

Department of Microbiology and Immunology, Emory University, Atlanta, Georgia 30322

Received 10 January 2000/Accepted 8 March 2000

Through cognate interaction between antigen-specific B-cell and CD4+ alpha beta T cells, the CD4+ alpha beta T cells secrete cytokines that initiate immunoglobulin (Ig) class switching from IgM to IgG. In this study, we show that formalin-inactivated influenza PR8 virus induces virus-specific IgM and IgG responses in the absence of CD4+ T cells and that all four subclasses of IgG are produced. The immunized CD4-deficient mice were also found to be completely protected against lethal infection with live, pathogenic influenza virus. The ability of CD4+ T-cell-deficient mice to generate these IgG responses was not found to be impaired when these mice were depleted of CD8+ T cells with an anti-CD8 monoclonal antibody. In contrast, alpha beta T-cell-deficient mice (TCRbeta -/-) were not found to produce significant amounts of IgG upon immunization with formalin-inactivated PR8 virus. These results suggest that CD4- CD8- double-negative alpha beta T cells are playing a role in regulating Ig class switching in the absence of CD4+ T cells.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, Emory University, Atlanta, GA 30322. Phone: (404) 727-5947. Fax: (404) 727-8250. E-mail: compans{at}microbio.emory.edu.

Journal of Virology, June 2000, p. 4999-5005, Vol. 74, No. 11
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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