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Journal of Virology, May 2000, p. 4908-4911, Vol. 74, No. 10
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Maternally Transferred Antibodies from DNA-Immunized Avians Protect Offspring against Hepadnavirus Infection

Christine Rollier, Celine Charollois, Catherine Jamard, Christian Trepo, and Lucyna Cova*

Unité de Recherche sur les Virus des Hépatites et les Pathologies Associées, Institut National de la Santé et de la Recherche Médicale, Unité 271, Lyon, France

Received 28 December 1999/Accepted 25 February 2000

The outcome and protective efficacy of maternal antibodies elicited by DNA immunization to the large (L) hepadnavirus envelope protein were studied using the duck hepatitis B virus (DHBV) model. Following genetic immunization of breeding ducks with a DHBV L protein gene-bearing plasmid, specific and highly neutralizing antibodies were transferred from the sera of immunized ducks, via the egg yolk, to the progeny of vaccinees. Interestingly, large amounts (60 to 100 mg/egg) of high-titer and L protein-specific yolk immunoglobulins (immunoglobulin Y) accumulated in the egg yolk. These results suggest that eggs from genetically immunized avians may represent a potent source of DNA-designed antibodies specific to viral antigen. Importantly, these antibodies are vertically transmitted and protect offspring against high-titer DHBV challenge.

* Corresponding author. Mailing address: Unité de Recherche sur les Virus des Hépatites et les Pathologies Associées, INSERM U271, 151 cours Albert Thomas, F-69424 Lyon Cedex 03, France. Phone: 33 4 72 68 19 81. Fax: 33 4 72 68 19 71. E-mail: cova{at}lyon151.inserm.fr.

Journal of Virology, May 2000, p. 4908-4911, Vol. 74, No. 10
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Thermet, A., Buronfosse, T., Werle-Lapostolle, B., Chevallier, M., Pradat, P., Trepo, C., Zoulim, F., Cova, L. (2008). DNA vaccination in combination or not with lamivudine treatment breaks humoral immune tolerance and enhances cccDNA clearance in the duck model of chronic hepatitis B virus infection. J. Gen. Virol. 89: 1192-1201 [Abstract] [Full Text]  
  • Thermet, A., Robaczewska, M., Rollier, C., Hantz, O., Trepo, C., Deleage, G., Cova, L. (2004). Identification of Antigenic Regions of Duck Hepatitis B Virus Core Protein with Antibodies Elicited by DNA Immunization and Chronic Infection. J. Virol. 78: 1945-1953 [Abstract] [Full Text]  
  • Zhang, Y.-Y., Summers, J. (2004). Rapid Production of Neutralizing Antibody Leads to Transient Hepadnavirus Infection. J. Virol. 78: 1195-1201 [Abstract] [Full Text]  


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