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Journal of Virology, May 2000, p. 4601-4611, Vol. 74, No. 10
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Isolation of Borna Disease Virus from Human Brain Tissue

Yurie Nakamura,1 Hirokazu Takahashi,1 Yuko Shoya,1 Takaaki Nakaya,1 Makiko Watanabe,2 Keizo Tomonaga,2 Kazuhiko Iwahashi,3 Kiyoshi Ameno,3 Noriko Momiyama,4 Hiroyuka Taniyama,4 Tetsutaro Sata,5 Takeshi Kurata,5 Juan Carlos de la Torre,6,* and Kazuyoshi Ikuta1,2,*

Section of Serology, Institute of Immunological Science, Hokkaido University, Kita-ku, Sapporo 060-0815,1 Department of Virology, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka 565-0871,2 Department of Neuropsychiatry, Kagawa Medical College, Kagawa 761-0007,3 Department of Pathology, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu 069-8501,4 and Department of Pathology, National Institute of Infectious Diseases, Shinjuku-ku, Tokyo 162-8640,5 Japan, and Department of Neuropharmacology, The Scripps Research Institute, La Jolla, California 920376

Received 16 November 1999/Accepted 4 February 2000

Serological and molecular epidemiological studies indicate that Borna disease virus (BDV) can infect humans and is possibly associated with certain neuropsychiatric disorders. We examined brain tissue collected at autopsy from four schizophrenic patients and two healthy controls for the presence of BDV markers in 12 different brain regions. BDV RNA and antigen was detected in four brain regions of a BDV-seropositive schizophrenic patient (P2) with a very recent (2 years) onset of disease. BDV markers exhibited a regionally localized distribution. BDV RNA was found in newborn Mongolian gerbils intracranially inoculated with homogenates from BDV-positive brain regions of P2. Human oligodendroglia (OL) cells inoculated with brain homogenates from BDV-positive gerbils allowed propagation and isolation of BDVHuP2br, a human brain-derived BDV. Virus isolation was also possible by transfection of Vero cells with ribonucleoprotein complexes prepared from BDV-positive human and gerbil brain tissues. BDVHuP2br was genetically closely related to but distinct from previously reported human- and animal-derived BDV sequences.

* Corresponding author. Mailing address for K. Ikuta: Department of Virology, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka 565-0871, Japan. Phone: 81 6 6879 8307. Fax: 81 6 6879 8310. E-mail: ikuta{at}biken.osaka-u.ac.jp. Mailing address for J. C. de la Torre: Department of Neuropharmacology, The Scripps Research Institute, 10550 N. Torrey Pines Rd., La Jolla, CA 92037. Phone: (858) 784-9462. Fax: (858) 784-9981. E-mail: juanct{at}scripps.edu.

Journal of Virology, May 2000, p. 4601-4611, Vol. 74, No. 10
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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