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Journal of Virology, January 2000, p. 523-528, Vol. 74, No. 1
0022-538X/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Small Dense Nuclear Bodies Are the Site of Localization of Herpes Simplex Virus 1 U_L3 and U_L4 Proteins and of ICP22 Only When the Latter Protein Is Present

Nancy S. Markovitz and Bernard Roizman^*

The Marjorie B. Kovler Viral Oncology Laboratories, The University of Chicago, Chicago, Illinois 60637

Received 5 August 1999/Accepted 1 October 1999

The herpes simplex virus 1 U_L3 and U_L4 open reading frames are expressed late in infection and are not essential for viral replication in cultured cells in vitro. An earlier report showed that the U_L4 protein colocalizes with the products of the 22/U_S1.5 genes in small nuclear dense bodies. Here we report that the U_L3 protein also colocalized in these small nuclear dense bodies and the localization of U_L3 and U_L4 proteins in these bodies required the presence of 22/U_S1.5 genes. In cells infected with a mutant lacking intact 22/U_S1.5 genes, U_L3 was diffused throughout the nucleus even though the overall accumulation of the 2 U_L3 protein was decreased. The results suggest that ICP22 acts both as a regulator of U_L3 accumulation and as the structural component and anchor of these small dense nuclear bodies.

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^* Corresponding author. Mailing address: The Marjorie B. Kovler Viral Oncology Laboratories, The University of Chicago, 910 E. 58th St., Chicago, IL 60637. Phone: (773) 702-1898. Fax: (773) 702-1631. E-mail: bernard{at}cummings.uchicago.edu.

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Journal of Virology, January 2000, p. 523-528, Vol. 74, No. 1
0022-538X/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

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