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Journal of Virology, January 2000, p. 493-504, Vol. 74, No. 1
0022-538X/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Structural Fingerprinting: Subgrouping of Comoviruses by Structural Studies of Red Clover Mottle Virus to 2.4-Å Resolution and Comparisons with Other Comovirusesdagger

Tianwei Lin,1 Anthony J. Clark,2,Dagger Zhongguo Chen,3,§ Michael Shanks,2 Jin-Bi Dai,3,∥ Ying Li,3,§ Tim Schmidt,3 Per Oxelfelt,4 George P. Lomonossoff,2 and John E. Johnson1,*

Department of Molecular Biology, The Scripps Research Institute, La Jolla, California 920371; Department of Virus Research, John Innes Centre, Norwich NR4 7UK, United Kingdom2; Structural Studies, Department of Biological Sciences, Purdue University, West Lafayette, Indiana 479073; and Department of Plant Pathology, Swedish University of Agricultural Sciences, S750 07 Uppsala, Sweden4

Received 12 April 1999/Accepted 20 September 1999

Red clover mottle virus (RCMV) is a member of the comoviruses, a group of picornavirus-like plant viruses. The X-ray structure of RCMV strain S has been determined and refined to 2.4 Å. The overall structure of RCMV is similar to that of two other comoviruses, Cowpea mosaic virus (CPMV) and Bean pod mottle virus (BPMV). The sequence of the coat proteins of RCMV strain O were modeled into the capsid structure of strain S without causing any distortion, confirming the close resemblance between the two strains. By comparing the RCMV structure with that of other comoviruses, a structural fingerprint at the N terminus of the small subunit was identified which allowed subgrouping of comoviruses into CPMV-like and BPMV-like viruses.

* Corresponding author. Mailing address: Department of Molecular Biology, The Scripps Research Institute, 10550 N. Torrey Pines Rd., La Jolla, CA 92037. Phone: (858) 784-2947. Fax: (858) 784-8660. E-mail: jackj{at}scripps.edu.

dagger Dedicated to the memory of J.-B. Dai.

Dagger Present address: Department of Plant Pathology, University of Kentucky, Lexington, KY 40546-0091.

§ Present address: Merck Research Laboratory, West Point, PA 19486.

∥ Deceased.

Journal of Virology, January 2000, p. 493-504, Vol. 74, No. 1
0022-538X/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

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