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Journal of Virology, September 1999, p. 7805-7811, Vol. 73, No. 9
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Intracellular Distribution of Rubella Virus Nonstructural Protein P150

Pekka Kujala,* Tero Ahola,dagger Neda Ehsani, Petri Auvinen, Helena Vihinen, and Leevi Kääriäinen

Institute of Biotechnology, University of Helsinki, Helsinki, Finland

Received 22 February 1999/Accepted 25 May 1999

Antiserum prepared against an amino-terminal fragment of rubella virus (RUB) nonstructural polyprotein was used to study RUB-infected Vero cells. Replicase protein P150 was associated with vesicles and vacuoles of endolysosomal origin and later with large, convoluted, tubular membrane structures. Newly incorporated bromouridine was associated with the same structures and specifically with small membrane invaginations, spherules, indicating that these structures may be the sites of viral RNA synthesis.

* Corresponding author. Mailing address: Institute of Biotechnology, Electronmicroscopy Unit, P.O. Box 56 (Viikinkaari 9), FIN-00014 University of Helsinki, Finland. Phone: 358-9-70859649. Fax: 358-9-70859560. E-mail: ptkujala{at}operoni.helsinki.fi.

dagger Present address: Institute for Molecular Virology, University of Wisconsin, Madison, WI 53706.

Journal of Virology, September 1999, p. 7805-7811, Vol. 73, No. 9
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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