This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Goepfert, P. A.
Right arrow Articles by Mulligan, M. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Goepfert, P. A.
Right arrow Articles by Mulligan, M. J.

 Previous Article  |  Next Article 

Journal of Virology, September 1999, p. 7210-7217, Vol. 73, No. 9
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

An Endoplasmic Reticulum Retrieval Signal Partitions Human Foamy Virus Maturation to Intracytoplasmic Membranes

Paul A. Goepfert,1,2,* Kit Shaw,2 George Wang,2 Anju Bansal,2 Bradley H. Edwards,1 and Mark J. Mulligan1,2

Departments of Medicine1 and Microbiology,2 University of Alabama at Birmingham, Birmingham, Alabama 35294-2170

Received 1 February 1999/Accepted 8 May 1999

Among all retroviruses, foamy viruses (FVs) are unique in that they regularly mature at intracytoplasmic membranes. The envelope glycoprotein of FV encodes an endoplasmic reticulum (ER) retrieval signal, the dilysine motif (KKXX), that functions to localize the human FV (HFV) glycoprotein to the ER. This study analyzed the function of the dilysine motif in the context of infectious molecular clones of HFV that encoded mutations in the dilysine motif. Electron microscopy (EM) demonstrated virion budding both intracytoplasmically and at the plasma membrane for the wild-type and mutant viruses. Additionally, mutant viruses retained their infectivity, but viruses lacking the dilysine signal budded at the plasma membrane to a greater extent than did wild-type viruses. Interestingly, this relative increase in budding across the plasma membrane did not increase the overall release of viral particles into cell culture media as measured by protein levels in viral pellets or infectious virus titers. We conclude that the dilysine motif of HFV imposes a partial restriction on the site of viral maturation but is not necessary for viral infectivity.

* Corresponding author. Mailing address: University of Alabama at Birmingham, Division of Infectious Diseases, BBRB 220, Birmingham, AL 35294-2170. Phone: (205) 975-5667. Fax: (205) 975-5718. E-mail: paulg{at}uab.edu.

Journal of Virology, September 1999, p. 7210-7217, Vol. 73, No. 9
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Patton, G. S., Morris, S. A., Chung, W., Bieniasz, P. D., McClure, M. O. (2005). Identification of Domains in Gag Important for Prototypic Foamy Virus Egress. J. Virol. 79: 6392-6399 [Abstract] [Full Text]  
  • Lontok, E., Corse, E., Machamer, C. E. (2004). Intracellular Targeting Signals Contribute to Localization of Coronavirus Spike Proteins near the Virus Assembly Site. J. Virol. 78: 5913-5922 [Abstract] [Full Text]  
  • Delelis, O., Saib, A., Sonigo, P. (2003). Biphasic DNA Synthesis in Spumaviruses. J. Virol. 77: 8141-8146 [Abstract] [Full Text]  
  • Shikova-Lekova, E., Lindemann, D., Pietschmann, T., Juretzek, T., Rudolph, W., Herchenroder, O., Gelderblom, H. R., Rethwilm, A. (2003). Replication-Competent Hybrids between Murine Leukemia Virus and Foamy Virus. J. Virol. 77: 7677-7681 [Abstract] [Full Text]  
  • Shaw, K. L., Lindemann, D., Mulligan, M. J., Goepfert, P. A. (2003). Foamy Virus Envelope Glycoprotein Is Sufficient for Particle Budding and Release. J. Virol. 77: 2338-2348 [Abstract] [Full Text]  
  • Lecellier, C.-H., Neves, M., Giron, M.-L., Tobaly-Tapiero, J., Saib, A. (2002). Further Characterization of Equine Foamy Virus Reveals Unusual Features among the Foamy Viruses. J. Virol. 76: 7220-7227 [Abstract] [Full Text]  
  • Eastman, S. W., Linial, M. L. (2001). Identification of a Conserved Residue of Foamy Virus Gag Required for Intracellular Capsid Assembly. J. Virol. 75: 6857-6864 [Abstract] [Full Text]  
  • Lindemann, D., Pietschmann, T., Picard-Maureau, M., Berg, A., Heinkelein, M., Thurow, J., Knaus, P., Zentgraf, H., Rethwilm, A. (2001). A Particle-Associated Glycoprotein Signal Peptide Essential for Virus Maturation and Infectivity. J. Virol. 75: 5762-5771 [Abstract] [Full Text]  
  • Meiering, C. D., Maxine L. Linial, (2001). Historical Perspective of Foamy Virus Epidemiology and Infection. Clin. Microbiol. Rev. 14: 165-176 [Abstract] [Full Text]  
  • Pietschmann, T., Zentgraf, H., Rethwilm, A., Lindemann, D. (2000). An Evolutionarily Conserved Positively Charged Amino Acid in the Putative Membrane-Spanning Domain of the Foamy Virus Envelope Protein Controls Fusion Activity. J. Virol. 74: 4474-4482 [Abstract] [Full Text]  
  • Tobaly-Tapiero, J., Bittoun, P., Neves, M., Guillemin, M.-C., Lecellier, C.-H., Puvion-Dutilleul, F., Gicquel, B., Zientara, S., Giron, M.-L., de Thé, H., Saïb, A. (2000). Isolation and Characterization of an Equine Foamy Virus. J. Virol. 74: 4064-4073 [Abstract] [Full Text]  
  • Ono, A., Orenstein, J. M., Freed, E. O. (2000). Role of the Gag Matrix Domain in Targeting Human Immunodeficiency Virus Type 1 Assembly. J. Virol. 74: 2855-2866 [Abstract] [Full Text]  
  • Bansal, A., Shaw, K. L., Edwards, B. H., Goepfert, P. A., Mulligan, M. J. (2000). Characterization of the R572T Point Mutant of a Putative Cleavage Site in Human Foamy Virus Env. J. Virol. 74: 2949-2954 [Abstract] [Full Text]  
  • Wang, G., Mulligan, M. J., Baldwin, D. N., Linial, M. L. (1999). Endogenous Virus of BHK-21 Cells Complicates Electron Microscopy Studies of Foamy Virus Maturation. J. Virol. 73: 8917-8917 [Full Text]  
  • Lambert, G., Becker, B., Schreiber, R., Boucherot, A., Reth, M., Kunzelmann, K. (2001). Control of Cystic Fibrosis Transmembrane Conductance Regulator Expression by BAP31. J. Biol. Chem. 276: 20340-20345 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»