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Journal of Virology, August 1999, p. 7031-7034, Vol. 73, No. 8
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

E4ORF3 Requirement for Achieving Long-Term Transgene Expression from the Cytomegalovirus Promoter in Adenovirus Vectors

Donna Armentano,1,* Michael P. Smith,1 Cathleen C. Sookdeo,1 Joseph Zabner,2 Michael A. Perricone,1 Judith A. St. George,1 Samuel C. Wadsworth,1 and Richard J. Gregory1

Genzyme Corporation, Framingham, Massachusetts 01701-9322,1 and Howard Hughes Medical Institute, College of Medicine, University of Iowa, Iowa City, Iowa 522422

Received 19 March 1999/Accepted 21 April 1999

Analysis of transgene expression under the control of the cytomegalovirus (CMV) promoter from adenovirus vectors in which the E4 region was modified indicated that E4ORF3 is required for long-term expression in the murine lung. CMV promoter truncation led to the persistence of expression in the absence of E4, thus eliminating the ORF3 requirement.

* Corresponding author. Mailing address: Genzyme Corporation, 31 New York Ave., Framingham, MA 01701-9322. Phone: (508) 872-8400, ext. 2402. Fax: (508) 872-4091. E-mail: darmentano{at}genzyme.com.

Journal of Virology, August 1999, p. 7031-7034, Vol. 73, No. 8
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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