This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lewin, S. R.
Right arrow Articles by Markowitz, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lewin, S. R.
Right arrow Articles by Markowitz, M.

 Previous Article  |  Next Article 

Journal of Virology, July 1999, p. 6099-6103, Vol. 73, No. 7
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Use of Real-Time PCR and Molecular Beacons To Detect Virus Replication in Human Immunodeficiency Virus Type 1-Infected Individuals on Prolonged Effective Antiretroviral Therapy

S. R. Lewin, M. Vesanen, L. Kostrikis, A. Hurley, M. Duran, L. Zhang, D. D. Ho, and M. Markowitz*

Aaron Diamond AIDS Research Center, New York, New York 10016

Received 25 November 1998/Accepted 5 March 1999

We have designed a novel, precise, and sensitive assay to measure unspliced (US) human immunodeficiency virus type 1 (HIV-1) mRNA in peripheral blood mononuclear cells of HIV-1-infected individuals by using real-time PCR and molecular beacons. Individuals were classified as either well suppressed (WS) or partially suppressed, based on longitudinal measurements of plasma HIV-1 RNA. The proportion of individuals with US mRNA undetectable over time was significantly higher among WS individuals; however, 30% of WS subjects still had detectable US mRNA after 24 months of effective antiviral therapy.

* Corresponding author. Mailing address: Aaron Diamond AIDS Research Center, 455 1st Ave., New York, NY 10016. Phone: (212) 448-5020. Fax: (212) 725-1126. E-mail: marty{at}adarc.org.

Journal of Virology, July 1999, p. 6099-6103, Vol. 73, No. 7
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Palmer, S., Maldarelli, F., Wiegand, A., Bernstein, B., Hanna, G. J., Brun, S. C., Kempf, D. J., Mellors, J. W., Coffin, J. M., King, M. S. (2008). Low-level viremia persists for at least 7 years in patients on suppressive antiretroviral therapy. Proc. Natl. Acad. Sci. USA 105: 3879-3884 [Abstract] [Full Text]  
  • Ellery, P. J., Tippett, E., Chiu, Y.-L., Paukovics, G., Cameron, P. U., Solomon, A., Lewin, S. R., Gorry, P. R., Jaworowski, A., Greene, W. C., Sonza, S., Crowe, S. M. (2007). The CD16+ Monocyte Subset Is More Permissive to Infection and Preferentially Harbors HIV-1 In Vivo. J. Immunol. 178: 6581-6589 [Abstract] [Full Text]  
  • Cameron, P. U., Handley, A. J., Baylis, D. C., Solomon, A. E., Bernard, N., Purcell, D. F. J., Lewin, S. R. (2007). Preferential Infection of Dendritic Cells during Human Immunodeficiency Virus Type 1 Infection of Blood Leukocytes. J. Virol. 81: 2297-2306 [Abstract] [Full Text]  
  • Harman, A. N., Wilkinson, J., Bye, C. R., Bosnjak, L., Stern, J. L., Nicholle, M., Lai, J., Cunningham, A. L. (2006). HIV Induces Maturation of Monocyte-Derived Dendritic Cells and Langerhans Cells. J. Immunol. 177: 7103-7113 [Abstract] [Full Text]  
  • Lee, W. A., He, G.-X., Eisenberg, E., Cihlar, T., Swaminathan, S., Mulato, A., Cundy, K. C. (2005). Selective Intracellular Activation of a Novel Prodrug of the Human Immunodeficiency Virus Reverse Transcriptase Inhibitor Tenofovir Leads to Preferential Distribution and Accumulation in Lymphatic Tissue. Antimicrob. Agents Chemother. 49: 1898-1906 [Abstract] [Full Text]  
  • Sharkey, M., Triques, K., Kuritzkes, D. R., Stevenson, M. (2005). In Vivo Evidence for Instability of Episomal Human Immunodeficiency Virus Type 1 cDNA. J. Virol. 79: 5203-5210 [Abstract] [Full Text]  
  • Kim, J. Y., Hwang, J.-M., Ko, H. S., Seong, M.-W., Park, B.-J., Park, S. S. (2005). Mitochondrial DNA content is decreased in autosomal dominant optic atrophy. Neurology 64: 966-972 [Abstract] [Full Text]  
  • Fondere, J.-M., Petitjean, G., Huguet, M.-F., Salhi, S. L., Baillat, V., Macura-Biegum, A., Becquart, P., Reynes, J., Vendrell, J.-P. (2004). Human Immunodeficiency Virus Type 1 (HIV-1) Antigen Secretion by Latently Infected Resting CD4+ T Lymphocytes from HIV-1-Infected Individuals. J. Virol. 78: 10536-10542 [Abstract] [Full Text]  
  • Di Mascio, M., Markowitz, M., Louie, M., Hurley, A., Hogan, C., Simon, V., Follmann, D., Ho, D. D., Perelson, A. S. (2004). Dynamics of Intermittent Viremia during Highly Active Antiretroviral Therapy in Patients Who Initiate Therapy during Chronic versus Acute and Early Human Immunodeficiency Virus Type 1 Infection. J. Virol. 78: 10566-10573 [Abstract] [Full Text]  
  • Lassen, K. G., Bailey, J. R., Siliciano, R. F. (2004). Analysis of Human Immunodeficiency Virus Type 1 Transcriptional Elongation in Resting CD4+ T Cells In Vivo. J. Virol. 78: 9105-9114 [Abstract] [Full Text]  
  • Wightman, F., Walters, T., Ayres, A., Bowden, S., Bartholomeusz, A., Lau, D., Locarnini, S., Lewin, S. R. (2004). Comparison of Sequence Analysis and a Novel Discriminatory Real-Time PCR Assay for Detection and Quantification of Lamivudine-Resistant Hepatitis B Virus Strains. J. Clin. Microbiol. 42: 3809-3812 [Abstract] [Full Text]  
  • Turville, S. G., Santos, J. J., Frank, I., Cameron, P. U., Wilkinson, J., Miranda-Saksena, M., Dable, J., Stossel, H., Romani, N., Piatak, M. Jr, Lifson, J. D., Pope, M., Cunningham, A. L. (2004). Immunodeficiency virus uptake, turnover, and 2-phase transfer in human dendritic cells. Blood 103: 2170-2179 [Abstract] [Full Text]  
  • Havlir, D. V., Strain, M. C., Clerici, M., Ignacio, C., Trabattoni, D., Ferrante, P., Wong, J. K. (2003). Productive Infection Maintains a Dynamic Steady State of Residual Viremia in Human Immunodeficiency Virus Type 1-Infected Persons Treated with Suppressive Antiretroviral Therapy for Five Years. J. Virol. 77: 11212-11219 [Abstract] [Full Text]  
  • Palmer, S., Wiegand, A. P., Maldarelli, F., Bazmi, H., Mican, J. M., Polis, M., Dewar, R. L., Planta, A., Liu, S., Metcalf, J. A., Mellors, J. W., Coffin, J. M. (2003). New Real-Time Reverse Transcriptase-Initiated PCR Assay with Single-Copy Sensitivity for Human Immunodeficiency Virus Type 1 RNA in Plasma. J. Clin. Microbiol. 41: 4531-4536 [Abstract] [Full Text]  
  • Weber, J., Rangel, H. R., Chakraborty, B., Tadele, M., Martinez, M. A., Martinez-Picado, J., Marotta, M. L., Mirza, M., Ruiz, L., Clotet, B., Wrin, T., Petropoulos, C. J., Quinones-Mateu, M. E. (2003). A novel TaqMan real-time PCR assay to estimate ex vivo human immunodeficiency virus type 1 fitness in the era of multi-target (pol and env) antiretroviral therapy. J. Gen. Virol. 84: 2217-2228 [Abstract] [Full Text]  
  • Hermankova, M., Siliciano, J. D., Zhou, Y., Monie, D., Chadwick, K., Margolick, J. B., Quinn, T. C., Siliciano, R. F. (2003). Analysis of Human Immunodeficiency Virus Type 1 Gene Expression in Latently Infected Resting CD4+ T Lymphocytes In Vivo. J. Virol. 77: 7383-7392 [Abstract] [Full Text]  
  • Spiess, B., Buchheidt, D., Baust, C., Skladny, H., Seifarth, W., Zeilfelder, U., Leib-Mosch, C., Morz, H., Hehlmann, R. (2003). Development of a LightCycler PCR Assay for Detection and Quantification of Aspergillus fumigatus DNA in Clinical Samples from Neutropenic Patients. J. Clin. Microbiol. 41: 1811-1818 [Abstract] [Full Text]  
  • Dworkin, L. L., Gibler, T. M., Van Gelder, R. N. (2002). Real-Time Quantitative Polymerase Chain Reaction Diagnosis of Infectious Posterior Uveitis. Arch Ophthalmol 120: 1534-1539 [Abstract] [Full Text]  
  • Limon, A., Nakajima, N., Lu, R., Ghory, H. Z., Engelman, A. (2002). Wild-Type Levels of Nuclear Localization and Human Immunodeficiency Virus Type 1 Replication in the Absence of the Central DNA Flap. J. Virol. 76: 12078-12086 [Abstract] [Full Text]  
  • Limon, A., Devroe, E., Lu, R., Ghory, H. Z., Silver, P. A., Engelman, A. (2002). Nuclear Localization of Human Immunodeficiency Virus Type 1 Preintegration Complexes (PICs): V165A and R166A Are Pleiotropic Integrase Mutants Primarily Defective for Integration, Not PIC Nuclear Import. J. Virol. 76: 10598-10607 [Abstract] [Full Text]  
  • Yun, Z., Fredriksson, E., Sonnerborg, A. (2002). Quantification of Human Immunodeficiency Virus Type 1 Proviral DNA by the TaqMan Real-Time PCR Assay. J. Clin. Microbiol. 40: 3883-3884 [Full Text]  
  • Butler, S. L., Johnson, E. P., Bushman, F. D. (2002). Human Immunodeficiency Virus cDNA Metabolism: Notable Stability of Two-Long Terminal Repeat Circles. J. Virol. 76: 3739-3747 [Abstract] [Full Text]  
  • Pierson, T. C., Kieffer, T. L., Ruff, C. T., Buck, C., Gange, S. J., Siliciano, R. F. (2002). Intrinsic Stability of Episomal Circles Formed during Human Immunodeficiency Virus Type 1 Replication. J. Virol. 76: 4138-4144 [Abstract] [Full Text]  
  • Weusten, J. J. A. M., Carpay, W. M., Oosterlaken, T. A. M., van Zuijlen, M. C. A., van de Wiel, P. A. (2002). Principles of quantitation of viral loads using nucleic acid sequence-based amplification in combination with homogeneous detection using molecular beacons. Nucleic Acids Res 30: e26-e26 [Abstract] [Full Text]  
  • Mackay, I. M., Arden, K. E., Nitsche, A. (2002). Real-time PCR in virology. Nucleic Acids Res 30: 1292-1305 [Abstract] [Full Text]  
  • Kedzierska, K., Ellery, P., Mak, J., Lewin, S. R., Crowe, S. M., Jaworowski, A. (2002). HIV-1 Down-Modulates {gamma} Signaling Chain of Fc{gamma}R in Human Macrophages: A Possible Mechanism for Inhibition of Phagocytosis. J. Immunol. 168: 2895-2903 [Abstract] [Full Text]  
  • Aldea, C., Alvarez, C. P., Folgueira, L., Delgado, R., Otero, J. R. (2002). Rapid Detection of Herpes Simplex Virus DNA in Genital Ulcers by Real-Time PCR Using SYBR Green I Dye as the Detection Signal. J. Clin. Microbiol. 40: 1060-1062 [Abstract] [Full Text]  
  • Zhu, T., Muthui, D., Holte, S., Nickle, D., Feng, F., Brodie, S., Hwangbo, Y., Mullins, J. I., Corey, L. (2002). Evidence for Human Immunodeficiency Virus Type 1 Replication In Vivo in CD14+ Monocytes and Its Potential Role as a Source of Virus in Patients on Highly Active Antiretroviral Therapy. J. Virol. 76: 707-716 [Abstract] [Full Text]  
  • Fang, Y., Wu, W.-H., Pepper, J. L., Larsen, J. L., Marras, S. A. E., Nelson, Eric. A., Epperson, W. B., Christopher-Hennings, J. (2002). Comparison of Real-Time, Quantitative PCR with Molecular Beacons to Nested PCR and Culture Methods for Detection of Mycobacterium avium subsp. paratuberculosis in Bovine Fecal Samples. J. Clin. Microbiol. 40: 287-291 [Abstract] [Full Text]  
  • Cole, S. W., Naliboff, B. D., Kemeny, M. E., Griswold, M. P., Fahey, J. L., Zack, J. A. (2001). Impaired response to HAART in HIV-infected individuals with high autonomic nervous system activity. Proc. Natl. Acad. Sci. USA 98: 12695-12700 [Abstract] [Full Text]  
  • Crowe, S. M., Sonza, S. (2000). HIV-1 can be recovered from a variety of cells including peripheral blood monocytes of patients receiving highly active antiretroviral therapy: a further obstacle to eradication. J. Leukoc. Biol. 68: 345-350 [Abstract] [Full Text]  
  • Patel, R., van Beuningen, R., van Strijp, D., Kurn, N. (2000). Quantification of HIV-1 RNA Using a Homogeneous Single-Tube Assay. Clin. Chem. 46: 1487-1488 [Full Text]  
  • Chen, Z., Huang, Y., Zhao, X., Skulsky, E., Lin, D., Ip, J., Gettie, A., Ho, D. D. (2000). Enhanced Infectivity of an R5-Tropic Simian/Human Immunodeficiency Virus Carrying Human Immunodeficiency Virus Type 1 Subtype C Envelope after Serial Passages in Pig-Tailed Macaques (Macaca nemestrina). J. Virol. 74: 6501-6510 [Abstract] [Full Text]  
  • Cimarelli, A., Sandin, S., Höglund, S., Luban, J. (2000). Rescue of Multiple Viral Functions by a Second-Site Suppressor of a Human Immunodeficiency Virus Type 1 Nucleocapsid Mutation. J. Virol. 74: 4273-4283 [Abstract] [Full Text]  
  • Oxenius, A., Price, D. A., Easterbrook, P. J., O'Callaghan, C. A., Kelleher, A. D., Whelan, J. A., Sontag, G., Sewell, A. K., Phillips, R. E. (2000). Early highly active antiretroviral therapy for acute HIV-1 infection preserves immune function of CD8+ and CD4+ T lymphocytes. Proc. Natl. Acad. Sci. USA 97: 3382-3387 [Abstract] [Full Text]  
  • Chakrabarti, L. A., Lewin, S. R., Zhang, L., Gettie, A., Luckay, A., Martin, L. N., Skulsky, E., Ho, D. D., Cheng-Mayer, C., Marx, P. A. (2000). Normal T-Cell Turnover in Sooty Mangabeys Harboring Active Simian Immunodeficiency Virus Infection. J. Virol. 74: 1209-1223 [Abstract] [Full Text]  
  • Markowitz, M. (2000). Resistance, Fitness, Adherence, and Potency: Mapping the Paths to Virologic Failure. JAMA 283: 250-251 [Full Text]  
  • Dornadula, G., Zhang, H., VanUitert, B., Stern, J., Livornese, L. Jr, Ingerman, M. J., Witek, J., Kedanis, R. J., Natkin, J., DeSimone, J., Pomerantz, R. J. (1999). Residual HIV-1 RNA in Blood Plasma of Patients Taking Suppressive Highly Active Antiretroviral Therapy. JAMA 282: 1627-1632 [Abstract] [Full Text]  
  • D'Aquila, R., Walker, B. (1999). Exploring the Benefits and Limits of Highly Active Antiretroviral Therapy. JAMA 282: 1668-1669 [Full Text]  
  • Chun, T.-W., Fauci, A. S. (1999). Latent reservoirs of HIV: Obstacles to the eradication of virus. Proc. Natl. Acad. Sci. USA 96: 10958-10961 [Full Text]  
  • Zhang, L., Lewin, S. R., Markowitz, M., Lin, H.-H., Skulsky, E., Karanicolas, R., He, Y., Jin, X., Tuttleton, S., Vesanen, M., Spiegel, H., Kost, R., van Lunzen, J., Stellbrink, H.-J., Wolinsky, S., Borkowsky, W., Palumbo, P., Kostrikis, L. G., Ho, D. D. (1999). Measuring Recent Thymic Emigrants in Blood of Normal and HIV-1-Infected Individuals before and after Effective Therapy. JEM 190: 725-732 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»