This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Zhang, J.
Right arrow Articles by Sapp, C. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zhang, J.
Right arrow Articles by Sapp, C. M.

 Previous Article  |  Next Article 

Journal of Virology, July 1999, p. 5912-5917, Vol. 73, No. 7
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Recombination between Two Identical Sequences within the Same Retroviral RNA Molecule

Jiayou Zhang* and Christy M. Sapp

Department of Microbiology and Immunology and Markey Cancer Center, University of Kentucky, Lexington, Kentucky 40536-0096

Received 10 November 1998/Accepted 12 March 1999

As a consequence of being diploid viruses, members of the Retroviridae have a high recombination rate. To measure recombination between two identical sequences within the same RNA molecule per round of retroviral replication cycle, a murine leukemia virus based vector (JZ442 + 3' Hyg) has been constructed. It carries a drug resistance gene, hyg, and a 290-bp repeat sequence of the 3' hyg gene inserted into the 3' untranslated region of the green fluorescent protein gene (gfp). Under fluorescence microscopy, Hygr cells containing the recombinant proviruses were clear, while a green color was observed in the drug-resistant cells carrying the parental proviruses. The rate of recombination was determined by the ratio of the number of clear colonies to the total number of Hygr colonies (green and clear colonies). The rate of recombination was found to be 62% by this method. The intermolecular recombination rate between an infectious virus bearing two copies of the 290-bp segment and a noninfectious chimeric RNA virus containing only a single copy of this sequence was also measured.

* Corresponding author. Mailing address: 206 Combs Research Bldg., University of Kentucky, 800 Rose St., Lexington, KY 40536-0096. Phone: (606) 257-4456. Fax: (606) 257-8940. E-mail: jzhan1{at}pop.uky.edu.

Journal of Virology, July 1999, p. 5912-5917, Vol. 73, No. 7
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Onafuwa-Nuga, A., Telesnitsky, A. (2009). The Remarkable Frequency of Human Immunodeficiency Virus Type 1 Genetic Recombination. Microbiol. Mol. Biol. Rev. 73: 451-480 [Abstract] [Full Text]  
  • Onafuwa, A., An, W., Robson, N. D., Telesnitsky, A. (2003). Human Immunodeficiency Virus Type 1 Genetic Recombination Is More Frequent Than That of Moloney Murine Leukemia Virus despite Similar Template Switching Rates. J. Virol. 77: 4577-4587 [Abstract] [Full Text]  
  • Li, T., Zhang, J. (2002). Intramolecular Recombinations of Moloney Murine Leukemia Virus Occur during Minus-Strand DNA Synthesis. J. Virol. 76: 9614-9623 [Abstract] [Full Text]  
  • Hwang, C. K., Svarovskaia, E. S., Pathak, V. K. (2001). Dynamic copy choice: Steady state between murine leukemia virus polymerase and polymerase-dependent RNase H activity determines frequency of in vivo template switching. Proc. Natl. Acad. Sci. USA 10.1073/pnas.221289898v1 [Abstract] [Full Text]  
  • Zhang, J., Ma, Y. (2001). Evidence for Retroviral Intramolecular Recombinations. J. Virol. 75: 6348-6358 [Abstract] [Full Text]  
  • Li, T., Zhang, J. (2001). Retroviral recombination is temperature dependent. J. Gen. Virol. 82: 1359-1364 [Abstract] [Full Text]  
  • Li, T., Zhang, J. (2000). Determination of the Frequency of Retroviral Recombination between Two Identical Sequences within a Provirus. J. Virol. 74: 7646-7650 [Abstract] [Full Text]  
  • Svarovskaia, E. S., Delviks, K. A., Hwang, C. K., Pathak, V. K. (2000). Structural Determinants of Murine Leukemia Virus Reverse Transcriptase That Affect the Frequency of Template Switching. J. Virol. 74: 7171-7178 [Abstract] [Full Text]  
  • Zhang, J., Tang, L.-Y., Li, T., Ma, Y., Sapp, C. M. (2000). Most Retroviral Recombinations Occur during Minus-Strand DNA Synthesis. J. Virol. 74: 2313-2322 [Abstract] [Full Text]  
  • Hwang, C. K., Svarovskaia, E. S., Pathak, V. K. (2001). Dynamic copy choice: Steady state between murine leukemia virus polymerase and polymerase-dependent RNase H activity determines frequency of in vivo template switching. Proc. Natl. Acad. Sci. USA 98: 12209-12214 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»