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Journal of Virology, June 1999, p. 5166-5171, Vol. 73, No. 6
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Intracisternal Type A Particle-Mediated Activation of the Notch4/int3 Gene in a Mouse Mammary Tumor: Generation of Truncated Notch4/int3 mRNAs by Retroviral Splicing Events

Jong-Seo Lee,1 Tatsuya Haruna,1 Akinori Ishimoto,1 Tasuku Honjo,2 and Shin-ichi Yanagawa1,*

Department of Viral Oncology, Institute for Virus Research,1 and Department of Medical Chemistry, Faculty of Medicine,2 Kyoto University, Sakyo-ku, Kyoto 606-8507, Japan

Received 17 August 1998/Accepted 8 March 1999

The int3 oncogene was discovered as a frequent target in mouse mammary tumor virus-induced mammary tumors and encodes the intracellular domain of a Notch4/int3 protein. In one spontaneous mammary tumor, no. 9, that developed in a BALB/c mouse, we have found an insertion of a 1.2-kb sequence, consisting of a 5' long terminal repeat and gag sequences of an intracisternal type A particle (IAP) as well as an extra copy of the Notch4/int3 genomic sequences containing exons 23 and 24, into the intron between exons 24 and 25 of the Notch4/int3 gene. In this tumor, unique splicing events between the IAP and the Notch4/int3 sequences generated two types of IAP-Notch4/int3 fusion transcripts encoding two different portions of the intracellular domain of Notch4/int3 proteins: one with a RAM domain and the other without. Interestingly, these two proteins showed different subcellular localizations in a mouse mammary epithelial cell line, HC-11.

* Corresponding author. Mailing address: Department of Viral Oncology, Institute for Virus Research, Kyoto University, Sakyo-ku, Kyoto 606-8507, Japan. Phone: 81-75-751-3996. Fax: 81-75-751-3995. E-mail: syanagaw{at}virus.kyoto-u.ac.jp.

Journal of Virology, June 1999, p. 5166-5171, Vol. 73, No. 6
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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