This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Butsch, M.
Right arrow Articles by Boris-Lawrie, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Butsch, M.
Right arrow Articles by Boris-Lawrie, K.

 Previous Article  |  Next Article 

Journal of Virology, June 1999, p. 4847-4855, Vol. 73, No. 6
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

The 5' RNA Terminus of Spleen Necrosis Virus Contains a Novel Posttranscriptional Control Element That Facilitates Human Immunodeficiency Virus Rev/RRE-Independent Gag Production

Melinda Butsch,1,2 Stacey Hull,1,3 Yalai Wang,1 Tiffiney M. Roberts,1,3 and Kathleen Boris-Lawrie1,3,*

Department of Veterinary Biosciences, Center for Retrovirus Research, Comprehensive Cancer Center,1 Ohio State Biochemistry Program,2 and Molecular, Cellular, Developmental Biology Graduate Program,3 The Ohio State University, Columbus, Ohio 43210-1093

Received 27 July 1998/Accepted 2 March 1999

Previous work has shown that spleen necrosis virus (SNV) long terminal repeats (LTRs) are associated with Rex/Rex-responsive element-independent expression of bovine leukemia virus RNA and supports the hypothesis that SNV RNA contains a cis-acting element that interacts with cellular Rex-like proteins. To test this hypothesis, the human immunodeficiency virus type 1 (HIV) Rev/RRE-dependent gag gene was used as a reporter to analyze various SNV sequences. Gag enzyme-linked immunosorbent assay and Western blot analyses reveal that HIV Gag production is enhanced at least 20,000-fold by the 5' SNV LTR in COS, D17, and 293 cells. Furthermore, SNV RU5 in the sense but not the antisense orientation is sufficient to confer Rev/RRE-independent expression onto a cytomegalovirus-gag plasmid. In contrast, the SNV 3' LTR and 3' untranslated sequence between env and the LTR did not support Rev-independent gag expression. Quantitative RNase protection assays indicate that the SNV 5' RNA terminus enhances cytoplasmic accumulation and polysome association of HIV unspliced and spliced transcripts. However, comparison of the absolute amounts of polysomal RNA indicates that polysome association is not sufficient to account for the significant increase in Gag production by the SNV sequences. Our analysis reveals that the SNV 5' RNA terminus contains a unique cis-acting posttranscriptional control element that interacts with hypothetical cellular Rev-like proteins to facilitate HIV RNA transport and efficient translation.

* Corresponding author. Mailing address: Department of Veterinary Biosciences, Center for Retrovirus Research, The Ohio State University, Columbus, OH 43210-1093. Phone: (614) 292-1392. Fax: (614) 292-6473. E-mail: boris-lawrie.1{at}osu.edu.

Journal of Virology, June 1999, p. 4847-4855, Vol. 73, No. 6
0022-538X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Hofacre, A., Nitta, T., Fan, H. (2009). Jaagsiekte Sheep Retrovirus Encodes a Regulatory Factor, Rej, Required for Synthesis of Gag Protein. J. Virol. 83: 12483-12498 [Abstract] [Full Text]  
  • Groom, H. C. T., Anderson, E. C., Lever, A. M. L. (2009). Rev: beyond nuclear export. J. Gen. Virol. 90: 1303-1318 [Abstract] [Full Text]  
  • Bolinger, C., Yilmaz, A., Hartman, T. R., Kovacic, M. B., Fernandez, S., Ye, J., Forget, M., Green, P. L., Boris-Lawrie, K. (2007). RNA helicase A interacts with divergent lymphotropic retroviruses and promotes translation of human T-cell leukemia virus type 1. Nucleic Acids Res 0: gkm124v1-14 [Abstract] [Full Text]  
  • Caporale, M., Cousens, C., Centorame, P., Pinoni, C., De las Heras, M., Palmarini, M. (2006). Expression of the jaagsiekte sheep retrovirus envelope glycoprotein is sufficient to induce lung tumors in sheep.. J. Virol. 80: 8030-8037 [Abstract] [Full Text]  
  • Parveen, Z., Mukhtar, M., Goodrich, A., Acheampong, E., Dornburg, R., Pomerantz, R. J. (2004). Cross-Packaging of Human Immunodeficiency Virus Type 1 Vector RNA by Spleen Necrosis Virus Proteins: Construction of a New Generation of Spleen Necrosis Virus-Derived Retroviral Vectors. J. Virol. 78: 6480-6488 [Abstract] [Full Text]  
  • Roberts, T. M., Boris-Lawrie, K. (2003). Primary Sequence and Secondary Structure Motifs in Spleen Necrosis Virus RU5 Confer Translational Utilization of Unspliced Human Immunodeficiency Virus Type 1 Reporter RNA. J. Virol. 77: 11973-11984 [Abstract] [Full Text]  
  • Wilson, C. A., Laeeq, S., Ritzhaupt, A., Colon-Moran, W., Yoshimura, F. K. (2002). Sequence Analysis of Porcine Endogenous Retrovirus Long Terminal Repeats and Identification of Transcriptional Regulatory Regions. J. Virol. 77: 142-149 [Abstract] [Full Text]  
  • Hull, S., Boris-Lawrie, K. (2002). RU5 of Mason-Pfizer Monkey Virus 5' Long Terminal Repeat Enhances Cytoplasmic Expression of Human Immunodeficiency Virus Type 1 gag-pol and Nonviral Reporter RNA. J. Virol. 76: 10211-10218 [Abstract] [Full Text]  
  • Dangel, A. W., Hull, S., Roberts, T. M., Boris-Lawrie, K. (2002). Nuclear Interactions Are Necessary for Translational Enhancement by Spleen Necrosis Virus RU5. J. Virol. 76: 3292-3300 [Abstract] [Full Text]  
  • Russell, R. A., Zeng, Y., Erlwein, O., Cullen, B. R., McClure, M. O. (2001). The R Region Found in the Human Foamy Virus Long Terminal Repeat Is Critical for both Gag and Pol Protein Expression. J. Virol. 75: 6817-6824 [Abstract] [Full Text]  
  • Guan, Y., Whitney, J. B., Detorio, M., Wainberg, M. A. (2001). Construction and In Vitro Properties of a Series of Attenuated Simian Immunodeficiency Viruses with All Accessory Genes Deleted. J. Virol. 75: 4056-4067 [Abstract] [Full Text]  
  • Dang, Q., Hu, W.-S. (2001). Effects of Homology Length in the Repeat Region on Minus-Strand DNA Transfer and Retroviral Replication. J. Virol. 75: 809-820 [Abstract] [Full Text]  
  • Butsch, M., Boris-Lawrie, K. (2000). Translation Is Not Required To Generate Virion Precursor RNA in Human Immunodeficiency Virus Type 1-Infected T Cells. J. Virol. 74: 11531-11537 [Abstract] [Full Text]  
  • Roberts, T. M., Boris-Lawrie, K. (2000). The 5' RNA Terminus of Spleen Necrosis Virus Stimulates Translation of Nonviral mRNA. J. Virol. 74: 8111-8118 [Abstract] [Full Text]  
  • Kucerova, L., Altanerova, V., Altaner, C., Boris-Lawrie, K. (1999). Bovine Leukemia Virus Structural Gene Vectors Are Immunogenic and Lack Pathogenicity in a Rabbit Model. J. Virol. 73: 8160-8166 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»